Cloning, Expression, and Functional Analysis of the MYB Transcription Factor SlMYB86-like in Tomato

MYB transcription factors (TFs) have been shown to play a key role in plant growth and development and are in response to various types of biotic and abiotic stress. Here, we clarified the structure, expression patterns, and function of a TF, (Solyc06g071690) in tomato using an inbred tomato line exhibiting high resistance to bacterial wilt (Hm 2-2 (R)) and one susceptible line (BY 1-2 (S)). The full-length cDNA sequence of this gene was 1226 bp, and the open reading frame was 966 bp, which encoded 321 amino acids; its relative molecular weight was 37.05055 kDa; its theoretical isoelectric point was 7.22; it was a hydrophilic nonsecreted protein; and it had no transmembrane structures. The protein also contains a highly conserved MYB DNA-binding domain and was predicted to be localized to the nucleus. Phylogenetic analysis revealed that SlMYB86-like is closely related to SpMYB86-like in and clustered with other members of the family . Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of the gene was tissue specific and could be induced by , salicylic acid, and jasmonic acid. The results of virus-induced gene silencing (VIGS) revealed that silencing decreased the resistance of tomato plants to bacterial wilt, suggesting that it positively regulates the resistance of tomatoes to bacterial wilt. Overall, these findings indicate that plays a key role in regulating the resistance of tomatoes to bacterial wilt.