Hepatitis B virus vaccination post serological testing and antibody levels of vaccinated health care workers in Accra, Ghana

•The seroprotection rate following HBV vaccination is 91.3 % among Health Care Workers in Ghana.•Only 21.3 % of vaccinated Health Care Workers tested for Anti-HBs.•Health facility level and risk perception for HBV infection are associated with post-vaccination serological testing. Hepatitis B Virus (HBV) infection is an important occupational hazard to Health Care Workers (HCWs) all over the world. International health organizations have strongly recommended the use of the HBV vaccine, especially among individuals at risk of HBV infection. A laboratory test aimed at measuring Anti-HBs concentration (titer) 1–2 months following a 3-dose vaccination schedule is the most reliable approach for diagnosing seroprotection against HBV. This study sought to assess post-vaccination serological testing, seroprotection against HBV, and associated factors among vaccinated HCWs in Ghana. A hospital-based analytical cross-sectional study involving 207 HCWs. Pretested questionnaires were used to collect data. 5mls of venous blood were collected from consenting HCWs under strict aseptic conditions and quantitatively analyzed for Anti-HBs using ELISA procedures. SPSS Version 23 was used to analyze data with the level of significance set at 0.05. Median age; 33, IQR of 29–39. The post-vaccination serological testing rate was 21.3 %. HCWs with high-risk perception and working at the regional hospital had lower odds of adherence to post-vaccination serological testing (aOR = 0.2; 95 % CI = 0.1–0.7) and (aOR = 0.1; 95 % CI = 0.1–0.6) p