Optimization ultrasonic-microwave-assisted extraction of phenolic compounds from Clinacanthus nutans using response surface methodology

Clinacanthus nutans (C. nutans) is an edible profitable herb with high phenolic content that recognized herb relieves skin disorder, antityrosinase, and anticancer. Along with these health benefits C. nutans, however, there is no study on the factors that influence the phenolic content of C. nutans extraction by water-based ultrasonic-microwave-assisted extraction (UMAE). The aim of this study evaluates UMAE conditions (ultrasonic power, microwave power, and extraction time) on responses using response surface Box-Behnken design and compared with the hydrothermal extraction. The findings found that the caffeic acid and ferulic acid content decrease with increasing the microwave power and long extraction time (P