Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomass

Brevibacterium sp. is commercially essential due to its production of amino acids, particularly glutamic acid and lysine. This work investigates the effect of Brevibacterium sp. concentration on L-amino acid synthesis from glucose hydrolysed from oil palm trunk biomass as a substrate. The study employed a two-factorial, completely randomised experimental design. Factor A consisted of concentrations of Brevibacterium sp. (1%, 3%, and 5%), while factor B was incubation duration (24 and 48 hours). The study measured the concentrations of four amino acids (alanine, glycine, tyrosine, and glutamic acid) using the colourimetric ninhydrin reaction. Additionally, the viability and antioxidant activity of Brevibacterium sp. during incubation time was measured with spectrophotometry (UV-VIS). The results indicate no significant interaction between factors (P>0.05) but a significant effect (P