Foliar spraying with bacterial biocontrol agents for the control of common bacterial blight of bean

The objective of this work was to evaluate the effect of foliar spraying with bacterial biocontrol agents (BBAs) on the control of common bacterial blight (CBB) of bean, and on the induction of systemic resistance in bean plants. CBB control by BBAs was evaluated by spraying bean leaves 48 and 24 ho...

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Veröffentlicht in:Pesquisa agropecuaria brasileira 2018-10, Vol.53 (10), p.1101-1108
Hauptverfasser: Sangiogo, Maurício, Rodriguez, Daniela Pimentel, Moccellin, Renata, Bermudez, Johan Manuel Murcia, Corrêa, Bianca Obes, Moura, Andrea Bittencourt
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Sprache:eng
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Zusammenfassung:The objective of this work was to evaluate the effect of foliar spraying with bacterial biocontrol agents (BBAs) on the control of common bacterial blight (CBB) of bean, and on the induction of systemic resistance in bean plants. CBB control by BBAs was evaluated by spraying bean leaves 48 and 24 hours before and after pathogen inoculation (BPI and API, respectively), with: DFs93, Bacillus cereus; DFs513, Pseudomonas veronii; DFs769, B. cereus; the C01 combination, DFs93 + DFs769 + DFs831 (Pseudomonas fluorescens); the C03 combination, DFs348 (Bacillus sp.) + DFs769 + DFs831; and water (control). Systemic effects were analyzed after spraying DFs513, DFs769, C03, and water 72 and 48 hours BPI. Phaseolin production induced by DFs348, DFs513, DFs769, DFs831, and water was also assessed. DFs513, DFs769, and C03 significantly reduced disease incidence (area under disease progress curve), regardless of spraying time and disease severity when sprayed 72 and 48 hours BPI. The DFs769 and DFs831 isolates induced the accumulation of phytoalexin (phaseolin). Therefore, DFs513, DFs769, and C03 show potential for the biocontrol of CBB when applied preventively on bean leaves, besides inducing systemic resistance. Resumo: O objetivo deste trabalho foi avaliar o efeito da pulverização foliar de agentes bacterianos de biocontrole (ABB) no controle do crestamento bacteriano comum do feijoeiro (CBC) e na indução de resistência sistêmica em plantas de feijão. O controle do CBC por ABB foi avaliado por meio da pulverização, em folhas de feijoeiro, 48 e 24 horas antes e depois da inoculação do patógeno (AIP e DIP, respectivamente), de: DFs93, Bacillus cereus; DFs513, Pseudomonas veronii; DFs769, B. cereus; combinação C01, DFs93 + DFs769 + DFs831 (Pseudomonas fluorescens); combinação C03, DFs348 (Bacillus sp.) + DFs769 + DFs831; e água destilada (testemunha). Os efeitos sistêmicos foram analisados após pulverização de DFs513, DFs769, C03 e água 48 e 72 horas AIP. Também foi avaliada a produção de faseolina induzida por DFs348, DFs513, DFs769, DFs831 e água. DFs513, DFs769 e C03 reduziram significativamente a incidência da doença (área abaixo da curva de progresso da doença), independentemente do momento da aplicação e da severidade da doença quando pulverizados 48 e 72 horas AIP. Os isolados DFs769 e DFs831 induziram o acúmulo de fitoalexina (faseolina). Portanto, DFs513, DFs769 e C03 apresentam potencial para o biocontrole do CBC quando pulverizados preventivamente em folhas de
ISSN:0100-204X
1678-3921
1678-3921
DOI:10.1590/s0100-204x2018001000003