Perspectivas de las ecuaciones biocinéticas y balance de materia para un biorreactor de flujo radial de células hepáticas

Bioreactors are one of the greatest solutions to the main diseases in Mexico in tissue engineering applications, they are used as a tool to propose different cell reproduction techniques as they provide different parameters. Biokinetic parameters are dictated by mathematics to provide operating conditions. This paper proposes a dynamic mathematical model design to determine the behavior of liver cells, protein and substrate production in a bioreactor. The proposed model returns as a result the prediction of performance of different variables over time. Relevant biokinetic parameters found are YXS = 2.94 g/g, YPS = 5.46 g/g, and μmáx = 1.84 1/h. Additionally, a parametric sensitivity analysis was executed to detect parameters that retain effects on biomass and product concentrations. The parameters are YXS, α, and β for biomass, and μmáx, YXS, and KPS for product concentrations. As an element of this study, diverse simulations were employed to determine performance at different initial substrate concentrations. The results establish that, the higher initial substrate concentration is, the lower the yield within the first eight days. Afterward yields are almost identical for all initial substrate concentrations.