Asynchrony between in vivo and in vitro rabbit embryos
Background: Comparative features of embryos developed under in vitro and in vivo conditions are particularly important in designing embryo transfer procedures that fulfil embryo-recipient synchronization requirements. Objective: To determine the degree of asynchrony in rabbit embryo development betw...
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| Veröffentlicht in: | Revista Colombiana de Ciencias Pecuarias 2022-04, Vol.35 (2), p.118-123 |
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| creator | García, María-Luz Belabbas, Rafik Muelas, Raquel Agea, Iván Argente, María-José |
| description | Background: Comparative features of embryos developed under in vitro and in vivo conditions are particularly important in designing embryo transfer procedures that fulfil embryo-recipient synchronization requirements. Objective: To determine the degree of asynchrony in rabbit embryo development between cultured and in vivo embryos. Methods: A total of 55 non-lactating multiparous female rabbits were used. Embryos were classified as 16-cells or early morulae at 48 hours post-coitum (hpc). Embryos were cultured during 30 or 32 h and embryo development was compared with in vivo embryos of 72 hpc. In vitro and in vivo embryos at 72 hpc were classified as early or compacted morulae. Bayesian statistics was used. Difference between in vivo and in vitro embryos and the actual probability of the difference between the in vivo and in vitro embryo higher than zero (P) was estimated. Results: The percentage of compacted morulae was higher in in vivo embryos than in in vitro embryos with +6 h of asynchrony (73.5 and 32.8%, P=1.00). But the percentage of compacted morulae was similar with +8 h asynchrony. Conclusions: In vitro embryos delay their development by + 8 hours compared to in vivo embryos. |
| doi_str_mv | 10.17533/udea.rccp.v35n2a02 |
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Objective: To determine the degree of asynchrony in rabbit embryo development between cultured and in vivo embryos. Methods: A total of 55 non-lactating multiparous female rabbits were used. Embryos were classified as 16-cells or early morulae at 48 hours post-coitum (hpc). Embryos were cultured during 30 or 32 h and embryo development was compared with in vivo embryos of 72 hpc. In vitro and in vivo embryos at 72 hpc were classified as early or compacted morulae. Bayesian statistics was used. Difference between in vivo and in vitro embryos and the actual probability of the difference between the in vivo and in vitro embryo higher than zero (P) was estimated. Results: The percentage of compacted morulae was higher in in vivo embryos than in in vitro embryos with +6 h of asynchrony (73.5 and 32.8%, P=1.00). But the percentage of compacted morulae was similar with +8 h asynchrony. Conclusions: In vitro embryos delay their development by + 8 hours compared to in vivo embryos.</description><identifier>ISSN: 0120-0690</identifier><identifier>EISSN: 2256-2958</identifier><identifier>DOI: 10.17533/udea.rccp.v35n2a02</identifier><language>eng</language><publisher>Medellín: Universidad de Antioquía</publisher><subject>Animal embryos ; asincronía embrionaria ; assincronia embrionária ; assisted reproduction ; Bayesian analysis ; coelho ; Coelhos ; conejo ; Conejos ; cultivo de embriones ; cultura de embriões ; Desarrollo embrionario ; desenvolvimento embrionário ; Embriones ; Embriões ; embryo culture ; embryo synchronization ; Embryo transfer ; embryonic asynchrony ; embryonic development ; Embryos ; in vitro embryo production ; in vivo embryo production ; In vivo methods and tests ; morula ; morulae ; mórula ; Producción de embriones in vitro ; producción de embriones in vivo ; Produção de embriões in vitro ; produção de embriões in vivo ; produção in vitro de embriões ; Rabbits ; Reproducción ; reproducción asistida ; Reproduction ; Reproductive technology ; Reprodução ; reprodução assistida ; sincronización de embriones ; sincronização de embriões ; Synchronism ; Synchronization ; Tecnologia reprodutiva ; Tecnología reproductiva ; Transferencia de embriones ; transferência de embrião</subject><ispartof>Revista Colombiana de Ciencias Pecuarias, 2022-04, Vol.35 (2), p.118-123</ispartof><rights>2022. This work is licensed under https://creativecommons.org/licenses/by-nc-sa/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,870,27903,27904</link.rule.ids></links><search><creatorcontrib>García, María-Luz</creatorcontrib><creatorcontrib>Belabbas, Rafik</creatorcontrib><creatorcontrib>Muelas, Raquel</creatorcontrib><creatorcontrib>Agea, Iván</creatorcontrib><creatorcontrib>Argente, María-José</creatorcontrib><title>Asynchrony between in vivo and in vitro rabbit embryos</title><title>Revista Colombiana de Ciencias Pecuarias</title><description>Background: Comparative features of embryos developed under in vitro and in vivo conditions are particularly important in designing embryo transfer procedures that fulfil embryo-recipient synchronization requirements. Objective: To determine the degree of asynchrony in rabbit embryo development between cultured and in vivo embryos. Methods: A total of 55 non-lactating multiparous female rabbits were used. Embryos were classified as 16-cells or early morulae at 48 hours post-coitum (hpc). Embryos were cultured during 30 or 32 h and embryo development was compared with in vivo embryos of 72 hpc. In vitro and in vivo embryos at 72 hpc were classified as early or compacted morulae. Bayesian statistics was used. Difference between in vivo and in vitro embryos and the actual probability of the difference between the in vivo and in vitro embryo higher than zero (P) was estimated. Results: The percentage of compacted morulae was higher in in vivo embryos than in in vitro embryos with +6 h of asynchrony (73.5 and 32.8%, P=1.00). But the percentage of compacted morulae was similar with +8 h asynchrony. Conclusions: In vitro embryos delay their development by + 8 hours compared to in vivo embryos.</description><subject>Animal embryos</subject><subject>asincronía embrionaria</subject><subject>assincronia embrionária</subject><subject>assisted reproduction</subject><subject>Bayesian analysis</subject><subject>coelho</subject><subject>Coelhos</subject><subject>conejo</subject><subject>Conejos</subject><subject>cultivo de embriones</subject><subject>cultura de embriões</subject><subject>Desarrollo embrionario</subject><subject>desenvolvimento embrionário</subject><subject>Embriones</subject><subject>Embriões</subject><subject>embryo culture</subject><subject>embryo synchronization</subject><subject>Embryo transfer</subject><subject>embryonic asynchrony</subject><subject>embryonic development</subject><subject>Embryos</subject><subject>in vitro embryo production</subject><subject>in vivo embryo production</subject><subject>In vivo methods and tests</subject><subject>morula</subject><subject>morulae</subject><subject>mórula</subject><subject>Producción de embriones in vitro</subject><subject>producción de embriones in vivo</subject><subject>Produção de embriões in vitro</subject><subject>produção de embriões in vivo</subject><subject>produção in vitro de embriões</subject><subject>Rabbits</subject><subject>Reproducción</subject><subject>reproducción asistida</subject><subject>Reproduction</subject><subject>Reproductive technology</subject><subject>Reprodução</subject><subject>reprodução assistida</subject><subject>sincronización de embriones</subject><subject>sincronização de embriões</subject><subject>Synchronism</subject><subject>Synchronization</subject><subject>Tecnologia reprodutiva</subject><subject>Tecnología reproductiva</subject><subject>Transferencia de embriones</subject><subject>transferência de embrião</subject><issn>0120-0690</issn><issn>2256-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><sourceid>FKZ</sourceid><sourceid>DOA</sourceid><recordid>eNpVkU-LFDEQxYMoOI5-Ai8NnnuspJJOB7wMi38WFgRZz6E6Xa0ZZpMx6RmZb2_vzih4quLx6lc8nhBvJWykNYjvjyPTpoRw2JzQJEWgnomVUqZrlTP9c7ECqaCFzsFL8arWHYCxPeJKdNt6TuFnyencDDz_Zk5NTM0pnnJDabzsc8lNoWGIc8MPQznn-lq8mGhf-c11rsX3Tx_vb760d18_395s79qgOjm3o0TSPepJy2nShieQbrBW9qCD65QZgh4BEINlNxBLazl0HfaAWmlJAdfi9sIdM-38ocQHKmefKfonIZcfnsocw569s4onBZPmQFpjGIgYxxGdcQGVsgvrw5UVaZ94_h931Y4plph35Ln67bd7AJAGlV6Aa_Hucn4o-deR6-x3-VjSkt4r65ZMGpVZXHhxhZJrLTz9-yLBP3XlH7vyj135v13hH7Uaibw</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>García, María-Luz</creator><creator>Belabbas, Rafik</creator><creator>Muelas, Raquel</creator><creator>Agea, Iván</creator><creator>Argente, María-José</creator><general>Universidad de Antioquía</general><general>Universidad de Antioquia</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>CLZPN</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>AGMXS</scope><scope>FKZ</scope><scope>DOA</scope></search><sort><creationdate>20220401</creationdate><title>Asynchrony between in vivo and in vitro rabbit embryos</title><author>García, María-Luz ; Belabbas, Rafik ; Muelas, Raquel ; Agea, Iván ; Argente, María-José</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c261t-d13a4834f41ff45ef019b771804c9625bc4d0033c7e9bae177ec6638034241ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal embryos</topic><topic>asincronía embrionaria</topic><topic>assincronia embrionária</topic><topic>assisted reproduction</topic><topic>Bayesian analysis</topic><topic>coelho</topic><topic>Coelhos</topic><topic>conejo</topic><topic>Conejos</topic><topic>cultivo de embriones</topic><topic>cultura de embriões</topic><topic>Desarrollo embrionario</topic><topic>desenvolvimento embrionário</topic><topic>Embriones</topic><topic>Embriões</topic><topic>embryo culture</topic><topic>embryo synchronization</topic><topic>Embryo transfer</topic><topic>embryonic asynchrony</topic><topic>embryonic development</topic><topic>Embryos</topic><topic>in vitro embryo production</topic><topic>in vivo embryo production</topic><topic>In vivo methods and tests</topic><topic>morula</topic><topic>morulae</topic><topic>mórula</topic><topic>Producción de embriones in vitro</topic><topic>producción de embriones in vivo</topic><topic>Produção de embriões in vitro</topic><topic>produção de embriões in vivo</topic><topic>produção in vitro de embriões</topic><topic>Rabbits</topic><topic>Reproducción</topic><topic>reproducción asistida</topic><topic>Reproduction</topic><topic>Reproductive technology</topic><topic>Reprodução</topic><topic>reprodução assistida</topic><topic>sincronización de embriones</topic><topic>sincronização de embriões</topic><topic>Synchronism</topic><topic>Synchronization</topic><topic>Tecnologia reprodutiva</topic><topic>Tecnología reproductiva</topic><topic>Transferencia de embriones</topic><topic>transferência de embrião</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García, María-Luz</creatorcontrib><creatorcontrib>Belabbas, Rafik</creatorcontrib><creatorcontrib>Muelas, Raquel</creatorcontrib><creatorcontrib>Agea, Iván</creatorcontrib><creatorcontrib>Argente, María-José</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>Latin America & Iberia Database</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Dialnet (Open Access Full Text)</collection><collection>Dialnet</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Revista Colombiana de Ciencias Pecuarias</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García, María-Luz</au><au>Belabbas, Rafik</au><au>Muelas, Raquel</au><au>Agea, Iván</au><au>Argente, María-José</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Asynchrony between in vivo and in vitro rabbit embryos</atitle><jtitle>Revista Colombiana de Ciencias Pecuarias</jtitle><date>2022-04-01</date><risdate>2022</risdate><volume>35</volume><issue>2</issue><spage>118</spage><epage>123</epage><pages>118-123</pages><issn>0120-0690</issn><eissn>2256-2958</eissn><abstract>Background: Comparative features of embryos developed under in vitro and in vivo conditions are particularly important in designing embryo transfer procedures that fulfil embryo-recipient synchronization requirements. Objective: To determine the degree of asynchrony in rabbit embryo development between cultured and in vivo embryos. Methods: A total of 55 non-lactating multiparous female rabbits were used. Embryos were classified as 16-cells or early morulae at 48 hours post-coitum (hpc). Embryos were cultured during 30 or 32 h and embryo development was compared with in vivo embryos of 72 hpc. In vitro and in vivo embryos at 72 hpc were classified as early or compacted morulae. Bayesian statistics was used. Difference between in vivo and in vitro embryos and the actual probability of the difference between the in vivo and in vitro embryo higher than zero (P) was estimated. Results: The percentage of compacted morulae was higher in in vivo embryos than in in vitro embryos with +6 h of asynchrony (73.5 and 32.8%, P=1.00). But the percentage of compacted morulae was similar with +8 h asynchrony. Conclusions: In vitro embryos delay their development by + 8 hours compared to in vivo embryos.</abstract><cop>Medellín</cop><pub>Universidad de Antioquía</pub><doi>10.17533/udea.rccp.v35n2a02</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal embryos asincronía embrionaria assincronia embrionária assisted reproduction Bayesian analysis coelho Coelhos conejo Conejos cultivo de embriones cultura de embriões Desarrollo embrionario desenvolvimento embrionário Embriones Embriões embryo culture embryo synchronization Embryo transfer embryonic asynchrony embryonic development Embryos in vitro embryo production in vivo embryo production In vivo methods and tests morula morulae mórula Producción de embriones in vitro producción de embriones in vivo Produção de embriões in vitro produção de embriões in vivo produção in vitro de embriões Rabbits Reproducción reproducción asistida Reproduction Reproductive technology Reprodução reprodução assistida sincronización de embriones sincronização de embriões Synchronism Synchronization Tecnologia reprodutiva Tecnología reproductiva Transferencia de embriones transferência de embrião |
| title | Asynchrony between in vivo and in vitro rabbit embryos |
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