First report of avocado tree wilt by Pythium sp. amazonianum in Mexico

Abstract In August and September 2014, different avocado orchards (Persea americana Mill. Var. Hass) were sampled in four municipalities (Tancítaro, Uruapan, San Juan Nuevo Parangaricutiro and Peribán) of the “Franja aguacatera” in Michoacan State, Mexico. Samples were collected from avocado trees s...

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Veröffentlicht in:Ciencia e investigación agraria 2018, Vol.45 (3), p.301-305
Hauptverfasser: Ochoa, Yisa María, Hernandez, Anselmo, Delgado, Juan Carlos, Beltran, Mariana, Tapia, Luis Mario, Hernandez, Omegar, Cerna, Ernesto
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container_end_page 305
container_issue 3
container_start_page 301
container_title Ciencia e investigación agraria
container_volume 45
creator Ochoa, Yisa María
Hernandez, Anselmo
Delgado, Juan Carlos
Beltran, Mariana
Tapia, Luis Mario
Hernandez, Omegar
Cerna, Ernesto
description Abstract In August and September 2014, different avocado orchards (Persea americana Mill. Var. Hass) were sampled in four municipalities (Tancítaro, Uruapan, San Juan Nuevo Parangaricutiro and Peribán) of the “Franja aguacatera” in Michoacan State, Mexico. Samples were collected from avocado trees showing symptoms of the “Tristeza del aguacate” disease. Strains of Pythium sp. were obtained consistently from symptomatic tissue isolated in the selective medium V8®-Agar-PARPH. Morphological identification was made through taxonomic keys, and molecular identification was obtained by sequencing Internal Transcribed Spacer (ITS) gene. Koch's postulates were corroborated by pathogenicity tests carried out on P. americana seedlings with six true leaves, identifying the strain of P. sp. amazonianum as the causal agent of the “Tristeza del aguacate” disease. This is the first report that associates the “Tristeza del aguacate” with P. sp. amazonianum in Michoacan, Mexico.
doi_str_mv 10.7764/rcia.v45i3.1905
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Inv. Agr</addtitle><description>Abstract In August and September 2014, different avocado orchards (Persea americana Mill. Var. Hass) were sampled in four municipalities (Tancítaro, Uruapan, San Juan Nuevo Parangaricutiro and Peribán) of the “Franja aguacatera” in Michoacan State, Mexico. Samples were collected from avocado trees showing symptoms of the “Tristeza del aguacate” disease. Strains of Pythium sp. were obtained consistently from symptomatic tissue isolated in the selective medium V8®-Agar-PARPH. Morphological identification was made through taxonomic keys, and molecular identification was obtained by sequencing Internal Transcribed Spacer (ITS) gene. Koch's postulates were corroborated by pathogenicity tests carried out on P. americana seedlings with six true leaves, identifying the strain of P. sp. amazonianum as the causal agent of the “Tristeza del aguacate” disease. 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subjects AGRICULTURE, MULTIDISCIPLINARY
Aguacate
Avocado
Michoacan
Michoacán
oomiceto
oomycete
pathogenicity
patogenicidad
Pythium
title First report of avocado tree wilt by Pythium sp. amazonianum in Mexico
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