Conservacao do semen da carpa comum, Cyprinus carpio, variedade ornamental, por resfriamento

The objective of this study was to assess the feasibility of semen preservation of common carp, ornamental variety, in the refrigerator at 4°C. Eight fish were used, pouring semen by abdominal massage. After application of crude extract of carp pituitary, the semen samples were extruded, and discarded the contaminated blood, feces and/or urine and showed that less than 80% motility. Each sample was divided into two tubes, one being maintained at room temperature (25 ± 3°C) and the other kept in the refrigerator (4 ± 2°C). For analysis of subjective motility, semen was pre-diluted by mixing one drop of semen + 4 drops of non-activating aqueous solution of NaCl 2%. The activation of the spermatozoa was performed by addition of 4 drops of distilled water to a drop of the mixture, since the microscope pre-focused. The analysis of sperm motility, both semen and kept at room temperature the stored under cooling, were taken every 1 hour. The motility of sperm (Y) is being reduced as a function of time held at room temperature (X) according to the equation: Y = 93.2 to 13.0. X (P