Comparative molecular analysis of endurance exercise in vivo with electrically stimulated in vitro myotube contraction - Supplementary Figure 3

Supplementary Figure 3. EPS condition similar to VWR did not enhance protein synthesis. A: The left and middle panel show a representative western blot using GA and TA muscle homogenates from sedentary and VWR mice (n = 6 per group). The right panel shows a representative western blot using lysates from C2C12 myotubes with EPS (n = 3). Western blotting was carried out using antibodies against p110, p85, pmTOR, mTOR, pS6K, S6K, p4E-BP1, 4E-BP1, pAkt (S473), pAkt (T308), Akt; α-tubulin antibodies served as a loading control. (B and C)