Additional file 6: of Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition

Figure S6. Impact of different concentrations of piperacillin on persister levels. Cultures at early stationary phase (t = 4 h) were treated with either 20 µg/mL piperacillin (A and B) or 50 µg/mL piperacillin (C and D). Control (untreated) was treated with equal volume of water. At t = 24 h, cells...

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Hauptverfasser:	Aedo, Sandra, Orman, Mehmet, Brynildsen, Mark
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Schlagworte:	Biochemistry Biophysics Biotechnology Cancer Cell Biology Developmental Biology FOS: Biological sciences FOS: Chemical sciences FOS: Clinical medicine FOS: Health sciences Immunology Infectious Diseases Inorganic Chemistry Microbiology Molecular Biology Pharmacology Plant Biology
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creator	Aedo, Sandra Orman, Mehmet Brynildsen, Mark
description	Figure S6. Impact of different concentrations of piperacillin on persister levels. Cultures at early stationary phase (t = 4 h) were treated with either 20 µg/mL piperacillin (A and B) or 50 µg/mL piperacillin (C and D). Control (untreated) was treated with equal volume of water. At t = 24 h, cells were washed, diluted into fresh media and treated with either 200 µg/mL ampicillin or 5 µg/mL ofloxacin. Survival fractions were monitored for 7 h. * p
doi_str_mv	10.6084/m9.figshare.8318972
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Impact of different concentrations of piperacillin on persister levels. Cultures at early stationary phase (t = 4 h) were treated with either 20 µg/mL piperacillin (A and B) or 50 µg/mL piperacillin (C and D). Control (untreated) was treated with equal volume of water. At t = 24 h, cells were washed, diluted into fresh media and treated with either 200 µg/mL ampicillin or 5 µg/mL ofloxacin. Survival fractions were monitored for 7 h. * p<0.05 (t-test). Data represent at least three biological replicates. Each data point was denoted as mean ± s.e. 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Impact of different concentrations of piperacillin on persister levels. Cultures at early stationary phase (t = 4 h) were treated with either 20 µg/mL piperacillin (A and B) or 50 µg/mL piperacillin (C and D). Control (untreated) was treated with equal volume of water. At t = 24 h, cells were washed, diluted into fresh media and treated with either 200 µg/mL ampicillin or 5 µg/mL ofloxacin. Survival fractions were monitored for 7 h. * p<0.05 (t-test). Data represent at least three biological replicates. Each data point was denoted as mean ± s.e. 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Impact of different concentrations of piperacillin on persister levels. Cultures at early stationary phase (t = 4 h) were treated with either 20 µg/mL piperacillin (A and B) or 50 µg/mL piperacillin (C and D). Control (untreated) was treated with equal volume of water. At t = 24 h, cells were washed, diluted into fresh media and treated with either 200 µg/mL ampicillin or 5 µg/mL ofloxacin. Survival fractions were monitored for 7 h. * p<0.05 (t-test). Data represent at least three biological replicates. Each data point was denoted as mean ± s.e. (TIF 151 kb)</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.8318972</doi><oa>free_for_read</oa></addata></record>
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subjects	Biochemistry Biophysics Biotechnology Cancer Cell Biology Developmental Biology FOS: Biological sciences FOS: Chemical sciences FOS: Clinical medicine FOS: Health sciences Immunology Infectious Diseases Inorganic Chemistry Microbiology Molecular Biology Pharmacology Plant Biology
title	Additional file 6: of Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition
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