(A and B) Day 8 differentiated ES cell cultures were reacted with the PECAM antibody, and representative areas were analyzed for vessel area (A) or branch point frequency (B)
(A) The mean vessel area of all rescue clones was significantly different from WT and mutant clones ( vs. sFlt and mFlt, P ≤ 0.0005; WT vs. sFlt and mFlt, P ≤ 0.02) but did not differ between sFlt-1 and mFlt-1 rescue clones (sFlt vs. mFlt, P = 0.86). (B) The mean branch points/millimeter vessel leng...
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