Reduced triosephosphate isomerase (TPI) activity increases oxidant resistance of and

The left panel shows a Western blot analysis of yeast cells expressing wild-type human TPI under the control of promoters of different strengths: (), (), and (). Yeast cells expressing human TPIor yeast TPI under the control of the strong promoter were used as controls. Equal loading of the lysates was controlled by visualizing G6PDH. The right panel shows yeast cells expressing yeast TPI and human TPIIle170Val controlled by the promoter or yeast expressing wild-type human TPI controlled by the , or promoters, respectively. Yeast were spotted as fivefold serial dilutions on SC medium supplemented with different concentrations of diamide. Plates were incubated at 30°C for 3 days. The left panel shows western blot analysis of cell extracts prepared from adult that were fed with producing double-stranded RNA of the gene (Y17G7B.7) (RNAi) or harboring the empty plasmid L4440 (control). The right panel shows the effects of the oxidants juglone and diamide on these worms. After feeding with as described above, worms were placed on agar plates supplemented with juglone or diamide. Multi-resistant (e1370) mutant worms were included in every experiment as controls.Copyright information:Taken from "Dynamic rerouting of the carbohydrate flux is key to counteracting oxidative stress"http://jbiol.com/content/6/4/10Journal of Biology 2007;6(4):10-10.Published online 21 Dec 2007PMCID:PMC2373902.