Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in...
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| creator | Guimarães, Andresa Raimundo, Juliana Macedo Rodrigues, Raisa Braul Maristela Peckle Peixoto Huarrisson Azevedo Santos André, Marcos Rogério Machado, Rosangela Zacarias Cristiane Divan Baldani |
| description | Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p |
| doi_str_mv | 10.6084/m9.figshare.7835300 |
| format | Dataset |
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Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.</description><identifier>DOI: 10.6084/m9.figshare.7835300</identifier><language>eng</language><publisher>SciELO journals</publisher><subject>Animal Physiology - Systems ; FOS: Biological sciences ; Parasitology</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,1888</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.7835300$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Guimarães, Andresa</creatorcontrib><creatorcontrib>Raimundo, Juliana Macedo</creatorcontrib><creatorcontrib>Rodrigues, Raisa Braul</creatorcontrib><creatorcontrib>Maristela Peckle Peixoto</creatorcontrib><creatorcontrib>Huarrisson Azevedo Santos</creatorcontrib><creatorcontrib>André, Marcos Rogério</creatorcontrib><creatorcontrib>Machado, Rosangela Zacarias</creatorcontrib><creatorcontrib>Cristiane Divan Baldani</creatorcontrib><title>Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil</title><description>Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.</description><subject>Animal Physiology - Systems</subject><subject>FOS: Biological sciences</subject><subject>Parasitology</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2019</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNo1z81OxCAYhWE2LszoFbjhAmylhRZY6mT8yyQmOq7JB3y1JG1pABd69WqcWZ13dZKHkKuG1T1T4mbW9RA-8ggJa6l4xxk7J--7MU3BjQFoXteahmVAV0Jcfov6OGMuwVEHJdMhxZm-hkg90mdYMKRI3woUvKY5fpYRIRd6l-A7TBfkbIAp4-VxN-RwvztsH6v9y8PT9nZfeaVZBdYy4TwXXFretFJyAYPE3vcWmGBMo-bcd620TdtbbJWTWnZeuU5IiVbxDeH_tx4KuFDQrCnMkL5Mw8yf2czanMzmaOY_8ntStg</recordid><startdate>20190313</startdate><enddate>20190313</enddate><creator>Guimarães, Andresa</creator><creator>Raimundo, Juliana Macedo</creator><creator>Rodrigues, Raisa Braul</creator><creator>Maristela Peckle Peixoto</creator><creator>Huarrisson Azevedo Santos</creator><creator>André, Marcos Rogério</creator><creator>Machado, Rosangela Zacarias</creator><creator>Cristiane Divan Baldani</creator><general>SciELO journals</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20190313</creationdate><title>Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil</title><author>Guimarães, Andresa ; Raimundo, Juliana Macedo ; Rodrigues, Raisa Braul ; Maristela Peckle Peixoto ; Huarrisson Azevedo Santos ; André, Marcos Rogério ; Machado, Rosangela Zacarias ; Cristiane Divan Baldani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d890-abb04cd3437b3127734af7e6d6ba04009e933d527b126be28c7975d8c5477eb83</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal Physiology - Systems</topic><topic>FOS: Biological sciences</topic><topic>Parasitology</topic><toplevel>online_resources</toplevel><creatorcontrib>Guimarães, Andresa</creatorcontrib><creatorcontrib>Raimundo, Juliana Macedo</creatorcontrib><creatorcontrib>Rodrigues, Raisa Braul</creatorcontrib><creatorcontrib>Maristela Peckle Peixoto</creatorcontrib><creatorcontrib>Huarrisson Azevedo Santos</creatorcontrib><creatorcontrib>André, Marcos Rogério</creatorcontrib><creatorcontrib>Machado, Rosangela Zacarias</creatorcontrib><creatorcontrib>Cristiane Divan Baldani</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Guimarães, Andresa</au><au>Raimundo, Juliana Macedo</au><au>Rodrigues, Raisa Braul</au><au>Maristela Peckle Peixoto</au><au>Huarrisson Azevedo Santos</au><au>André, Marcos Rogério</au><au>Machado, Rosangela Zacarias</au><au>Cristiane Divan Baldani</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil</title><date>2019-03-13</date><risdate>2019</risdate><abstract>Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.</abstract><pub>SciELO journals</pub><doi>10.6084/m9.figshare.7835300</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animal Physiology - Systems FOS: Biological sciences Parasitology |
| title | Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
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