Additional file 3: of Immunotherapy of triple-negative breast cancer with cathepsin D-targeting antibodies
Figure S2. Schematic overview of the biotinylation method used for the identification of accessible proteins in TNBC samples.The technique involves the biotinylation of TNBC biopsies by immersion in the chemically modified biotin solution. Proteins are then solubilized and biotinylated proteins are...
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| creator | Ashraf, Yahya Mansouri, Hanane Laurent-Matha, Valérie Alcaraz, Lindsay Roger, Pascal Guiu, Séverine Derocq, Danielle Robin, Gautier Henri-Alexandre Michaud Helène Delpech Jarlier, Marta Pugnière, Martine Robert, Bruno Puel, Anthony Martin, Lucie Landomiel, Flavie Bourquard, Thomas Achour, Oussama Fruitier-Arnaudin, Ingrid Pichard, Alexandre Deshayes, Emmanuel Turtoi, Andrei Poupon, Anne Simony-Lafontaine, Joëlle Boissière-Michot, Florence Pirot, Nelly Bernex, Florence Jacot, William Manoir, Stanislas Theillet, Charles Pouget, Jean-Pierre Navarro-Teulon, Isabelle Bonnefoy, Nathalie Pèlegrin, André Chardès, Thierry Martineau, Pierre Liaudet-Coopman, Emmanuelle |
| description | Figure S2. Schematic overview of the biotinylation method used for the identification of accessible proteins in TNBC samples.The technique involves the biotinylation of TNBC biopsies by immersion in the chemically modified biotin solution. Proteins are then solubilized and biotinylated proteins are captured on the streptavidin material. After their enrichment, these proteins can be analyzed using HPLC chromatographic separation, MS analysis, selection of a particular mass (peptide), and fragmentation (MS/MS). MS/MS yields a pattern that delivers the sequence of the peptides and contributes to protein identification. The putative biomarkers discovered by this method require subsequent validation, for instance by immunohistochemistry. (PPTX 42 kb) |
| doi_str_mv | 10.6084/m9.figshare.7672529 |
| format | Video |
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Schematic overview of the biotinylation method used for the identification of accessible proteins in TNBC samples.The technique involves the biotinylation of TNBC biopsies by immersion in the chemically modified biotin solution. Proteins are then solubilized and biotinylated proteins are captured on the streptavidin material. After their enrichment, these proteins can be analyzed using HPLC chromatographic separation, MS analysis, selection of a particular mass (peptide), and fragmentation (MS/MS). MS/MS yields a pattern that delivers the sequence of the peptides and contributes to protein identification. The putative biomarkers discovered by this method require subsequent validation, for instance by immunohistochemistry. (PPTX 42 kb)</description><identifier>DOI: 10.6084/m9.figshare.7672529</identifier><language>eng</language><publisher>figshare</publisher><subject>Biochemistry ; Biophysics ; Biotechnology ; Cell Biology ; Chemical Sciences not elsewhere classified ; Developmental Biology ; FOS: Chemical sciences ; FOS: Clinical medicine ; Immunology ; Inorganic Chemistry ; Molecular Biology</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,1887</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.7672529$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Ashraf, Yahya</creatorcontrib><creatorcontrib>Mansouri, Hanane</creatorcontrib><creatorcontrib>Laurent-Matha, Valérie</creatorcontrib><creatorcontrib>Alcaraz, Lindsay</creatorcontrib><creatorcontrib>Roger, Pascal</creatorcontrib><creatorcontrib>Guiu, Séverine</creatorcontrib><creatorcontrib>Derocq, Danielle</creatorcontrib><creatorcontrib>Robin, Gautier</creatorcontrib><creatorcontrib>Henri-Alexandre Michaud</creatorcontrib><creatorcontrib>Helène Delpech</creatorcontrib><creatorcontrib>Jarlier, Marta</creatorcontrib><creatorcontrib>Pugnière, Martine</creatorcontrib><creatorcontrib>Robert, Bruno</creatorcontrib><creatorcontrib>Puel, Anthony</creatorcontrib><creatorcontrib>Martin, Lucie</creatorcontrib><creatorcontrib>Landomiel, Flavie</creatorcontrib><creatorcontrib>Bourquard, Thomas</creatorcontrib><creatorcontrib>Achour, Oussama</creatorcontrib><creatorcontrib>Fruitier-Arnaudin, Ingrid</creatorcontrib><creatorcontrib>Pichard, Alexandre</creatorcontrib><creatorcontrib>Deshayes, Emmanuel</creatorcontrib><creatorcontrib>Turtoi, Andrei</creatorcontrib><creatorcontrib>Poupon, Anne</creatorcontrib><creatorcontrib>Simony-Lafontaine, Joëlle</creatorcontrib><creatorcontrib>Boissière-Michot, Florence</creatorcontrib><creatorcontrib>Pirot, Nelly</creatorcontrib><creatorcontrib>Bernex, Florence</creatorcontrib><creatorcontrib>Jacot, William</creatorcontrib><creatorcontrib>Manoir, Stanislas</creatorcontrib><creatorcontrib>Theillet, Charles</creatorcontrib><creatorcontrib>Pouget, Jean-Pierre</creatorcontrib><creatorcontrib>Navarro-Teulon, Isabelle</creatorcontrib><creatorcontrib>Bonnefoy, Nathalie</creatorcontrib><creatorcontrib>Pèlegrin, André</creatorcontrib><creatorcontrib>Chardès, Thierry</creatorcontrib><creatorcontrib>Martineau, Pierre</creatorcontrib><creatorcontrib>Liaudet-Coopman, Emmanuelle</creatorcontrib><title>Additional file 3: of Immunotherapy of triple-negative breast cancer with cathepsin D-targeting antibodies</title><description>Figure S2. Schematic overview of the biotinylation method used for the identification of accessible proteins in TNBC samples.The technique involves the biotinylation of TNBC biopsies by immersion in the chemically modified biotin solution. Proteins are then solubilized and biotinylated proteins are captured on the streptavidin material. After their enrichment, these proteins can be analyzed using HPLC chromatographic separation, MS analysis, selection of a particular mass (peptide), and fragmentation (MS/MS). MS/MS yields a pattern that delivers the sequence of the peptides and contributes to protein identification. The putative biomarkers discovered by this method require subsequent validation, for instance by immunohistochemistry. (PPTX 42 kb)</description><subject>Biochemistry</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Chemical Sciences not elsewhere classified</subject><subject>Developmental Biology</subject><subject>FOS: Chemical sciences</subject><subject>FOS: Clinical medicine</subject><subject>Immunology</subject><subject>Inorganic Chemistry</subject><subject>Molecular Biology</subject><fulltext>true</fulltext><rsrctype>video</rsrctype><creationdate>2019</creationdate><recordtype>video</recordtype><sourceid>PQ8</sourceid><recordid>eNqdjksOgkAQRGfjwqgncNMXAEH84c74ie7dTxpooA0zkJlWw-3VRC_gqiqVV8lTahpH4SraLGYmDUuufI2OwvVqPV_O06G67YqChVuLDZTcECRbaEu4GHO3rdTksOs_gzjuGgosVSj8IMgcoRfI0ebk4MlSv_ub7zxbOASCriJhWwFa4awtmPxYDUpsPE2-OVLJ6Xjdn4MCBXMW0p1jg67XcaQ_xtqk-mesv8bJf68X--dVxQ</recordid><startdate>20190205</startdate><enddate>20190205</enddate><creator>Ashraf, Yahya</creator><creator>Mansouri, Hanane</creator><creator>Laurent-Matha, Valérie</creator><creator>Alcaraz, Lindsay</creator><creator>Roger, Pascal</creator><creator>Guiu, Séverine</creator><creator>Derocq, Danielle</creator><creator>Robin, Gautier</creator><creator>Henri-Alexandre Michaud</creator><creator>Helène Delpech</creator><creator>Jarlier, Marta</creator><creator>Pugnière, Martine</creator><creator>Robert, Bruno</creator><creator>Puel, Anthony</creator><creator>Martin, Lucie</creator><creator>Landomiel, Flavie</creator><creator>Bourquard, Thomas</creator><creator>Achour, Oussama</creator><creator>Fruitier-Arnaudin, Ingrid</creator><creator>Pichard, Alexandre</creator><creator>Deshayes, Emmanuel</creator><creator>Turtoi, Andrei</creator><creator>Poupon, Anne</creator><creator>Simony-Lafontaine, Joëlle</creator><creator>Boissière-Michot, Florence</creator><creator>Pirot, Nelly</creator><creator>Bernex, Florence</creator><creator>Jacot, William</creator><creator>Manoir, Stanislas</creator><creator>Theillet, Charles</creator><creator>Pouget, Jean-Pierre</creator><creator>Navarro-Teulon, Isabelle</creator><creator>Bonnefoy, Nathalie</creator><creator>Pèlegrin, André</creator><creator>Chardès, Thierry</creator><creator>Martineau, Pierre</creator><creator>Liaudet-Coopman, Emmanuelle</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20190205</creationdate><title>Additional file 3: of Immunotherapy of triple-negative breast cancer with cathepsin D-targeting antibodies</title><author>Ashraf, Yahya ; 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Schematic overview of the biotinylation method used for the identification of accessible proteins in TNBC samples.The technique involves the biotinylation of TNBC biopsies by immersion in the chemically modified biotin solution. Proteins are then solubilized and biotinylated proteins are captured on the streptavidin material. After their enrichment, these proteins can be analyzed using HPLC chromatographic separation, MS analysis, selection of a particular mass (peptide), and fragmentation (MS/MS). MS/MS yields a pattern that delivers the sequence of the peptides and contributes to protein identification. The putative biomarkers discovered by this method require subsequent validation, for instance by immunohistochemistry. (PPTX 42 kb)</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.7672529</doi><oa>free_for_read</oa></addata></record> |
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| title | Additional file 3: of Immunotherapy of triple-negative breast cancer with cathepsin D-targeting antibodies |
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