Cloning, characterization, and expression of microRNAs from the Asian malaria mosquito, -1

Ed from the genome assembly. Alignment of the pre-miRNA hairpins found in and . The mature miRNA is marked in red while miRNA* is marked in blue. Conserved nucleotides are indicated by a "+". Hairpin structure of mir-76. The mature miRNA predicted by miRscan (Table 2) is shown in red. RPA analysis of ast-mir-76. Lane 1, RNA with probe and digested; Lane 2, yeast RNA with probe and digested; Lane 3, yeast RNA without probe and digested; Lanes 4 and 5, empty lanes; Lane 6, undigested probe. A band of the correct size was only observed in total RNA samples (Lane 1). The size of the protected RNA product in lane 1 was estimated to be 24 nucleotides using markers as described in Figure 1. This size is as expected (the protected 20-nt long ast-miR-76 plus 4 undigested adenosines, see Methods).Copyright information:Taken from "Cloning, characterization, and expression of microRNAs from the Asian malaria mosquito, "http://www.biomedcentral.com/1471-2164/9/244BMC Genomics 2008;9():244-244.Published online 23 May 2008PMCID:PMC2430712.