MOESM4 of Complement factors C3a and C5a mimick a proinflammatory microenvironment and increase HBV IGRA sensitivity

Additional file 4: Figure S4. Gating strategy for flow cytometric analysis of APC activation state. Gating strategy used for flow cytometric analysis of APCs upon stimulation of whole blood with HBsAg and HBsAg combined with C3a and C5a, respectively. First, dead cells and cell doublets were exclude...

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Bibliographische Detailangaben
Hauptverfasser:	Bröker, Katharina, Terzenbach, Robin, Bentzien, Frank, Lüth, Stefan, Dammermann, Werner
Format:	Bild
Sprache:	eng
Schlagworte:	Biochemistry Cancer Cell Biology Chemical Sciences not elsewhere classified Computational Biology Developmental Biology FOS: Biological sciences FOS: Chemical sciences Hematology Inorganic Chemistry Medicine Molecular Biology Plant Biology
Online-Zugang:	Volltext bestellen
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Zusammenfassung:	Additional file 4: Figure S4. Gating strategy for flow cytometric analysis of APC activation state. Gating strategy used for flow cytometric analysis of APCs upon stimulation of whole blood with HBsAg and HBsAg combined with C3a and C5a, respectively. First, dead cells and cell doublets were excluded. Within the MHCI+ population MCHII+ APCs were then identified and analyzed for expression of CD80 and CD86. Gates were set according to FMO controls. n = 5.
DOI:	10.6084/m9.figshare.7542623