Kinetics of surface expression of CD154 in stimulated T lymphocytes CD154 kinetic expression in CD4T lymphocytes from patients with rheumatoid arthritis (RA) after stimulation with anti-CD3/anti-CD28, according to genotype

Copyright information:Taken from "Association of the microsatellite in the 3' untranslated region of the gene with rheumatoid arthritis in females from a Spanish cohort: a case-control study"http://arthritis-research.com/content/9/5/R89Arthritis Research & Therapy 2007;9(5):R89-R89.Published online 10 Sep 2007PMCID:PMC2212561. Peripheral blood mononuclear cells from patients with RA (group, = 19; non-group, = 16) were stimulated with anti-CD3/anti-CD28 for 24, 48, 72, and 92 hours. After this, the surface expression of CD154 was measured by flow cytometry. The graph shows the median percentage of CD154 expression in CD4T lymphocytes from each group at the indicated times. *= 0.046, comparing the group with the non-group. Cytometry histograms showing CD154 staining (thick line) compared with non-specific staining (isotype control mAb, thin line) in CD4T lymphocytes from representative patients with RA (upper panel, ; lower panels, non-), after 24 hours (left panels) and 48 hours (right panels) of stimulation with anti-CD3/anti-CD28. PE, phycoerythrin.