COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma
Abstract Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC i...
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creator | Pinheiro, Danilo Do Rosário Harada, Maria Lucia Rommel Mario Rodriguez Burbano Borges, Barbara Do Nascimento |
description | Abstract Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 samples were obtained. A 675 bp fragment of the S. apella COX-2 promoter region was obtained, and it was 99.2% and 68.2% similar to H. sapiens and S. boliviensis, respectively. Similar to humans, several transcription factors and cis-regulatory element binding sites were identified in the S. apella sequence. MSP revealed that all samples were methylated. However, IHQ results demonstrated positive COX-2 expression in all pre-neoplastic and tumoral samples. The results suggest that the analyzed fragment is not crucial in COX-2 regulation of GC in S. apella. |
doi_str_mv | 10.6084/m9.figshare.6693476 |
format | Dataset |
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There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 samples were obtained. A 675 bp fragment of the S. apella COX-2 promoter region was obtained, and it was 99.2% and 68.2% similar to H. sapiens and S. boliviensis, respectively. Similar to humans, several transcription factors and cis-regulatory element binding sites were identified in the S. apella sequence. MSP revealed that all samples were methylated. However, IHQ results demonstrated positive COX-2 expression in all pre-neoplastic and tumoral samples. The results suggest that the analyzed fragment is not crucial in COX-2 regulation of GC in S. apella.</description><identifier>DOI: 10.6084/m9.figshare.6693476</identifier><language>eng</language><publisher>SciELO journals</publisher><subject>Biochemistry and Cell Biology not elsewhere classified ; FOS: Biological sciences ; Genetics not elsewhere classified</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1894</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.6693476$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Pinheiro, Danilo Do Rosário</creatorcontrib><creatorcontrib>Harada, Maria Lucia</creatorcontrib><creatorcontrib>Rommel Mario Rodriguez Burbano</creatorcontrib><creatorcontrib>Borges, Barbara Do Nascimento</creatorcontrib><title>COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma</title><description>Abstract Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 samples were obtained. A 675 bp fragment of the S. apella COX-2 promoter region was obtained, and it was 99.2% and 68.2% similar to H. sapiens and S. boliviensis, respectively. Similar to humans, several transcription factors and cis-regulatory element binding sites were identified in the S. apella sequence. MSP revealed that all samples were methylated. However, IHQ results demonstrated positive COX-2 expression in all pre-neoplastic and tumoral samples. The results suggest that the analyzed fragment is not crucial in COX-2 regulation of GC in S. apella.</description><subject>Biochemistry and Cell Biology not elsewhere classified</subject><subject>FOS: Biological sciences</subject><subject>Genetics not elsewhere classified</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2018</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNo1kL9uwyAYxL10qNI-QRdewC7GFOOxivpPipShGbKhz_DhUGGwgErN2zdW0-nuhjudflX10NJGUMkf56GxbsonSNgIMXS8F7dV3u6PNSMTBiT4syTM2cVAIBgyYzmdPZQ1Lyla55G4QD5hga_vTGBB74HAxYW1isnNGAp4MkeDntiYyAS5JKcJGAxRQ9IuxBnuqhsLPuP9VTfV4fXlsH2vd_u3j-3zrjZyELWxhmIPsheypXQADZ3ouRFa9nakrEU-MDHKkQuOjNlx1PAkKZU4Wsl7ybpN1f3NGiigXUG1XC5COquWqpWImgf1T0RdiXS_pHFfCw</recordid><startdate>20180627</startdate><enddate>20180627</enddate><creator>Pinheiro, Danilo Do Rosário</creator><creator>Harada, Maria Lucia</creator><creator>Rommel Mario Rodriguez Burbano</creator><creator>Borges, Barbara Do Nascimento</creator><general>SciELO journals</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20180627</creationdate><title>COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma</title><author>Pinheiro, Danilo Do Rosário ; Harada, Maria Lucia ; Rommel Mario Rodriguez Burbano ; Borges, Barbara Do Nascimento</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d896-dfd0e7a87681009aca3674d6c87fb021e4926b8b464e22fbbca58008ebf847823</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biochemistry and Cell Biology not elsewhere classified</topic><topic>FOS: Biological sciences</topic><topic>Genetics not elsewhere classified</topic><toplevel>online_resources</toplevel><creatorcontrib>Pinheiro, Danilo Do Rosário</creatorcontrib><creatorcontrib>Harada, Maria Lucia</creatorcontrib><creatorcontrib>Rommel Mario Rodriguez Burbano</creatorcontrib><creatorcontrib>Borges, Barbara Do Nascimento</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Pinheiro, Danilo Do Rosário</au><au>Harada, Maria Lucia</au><au>Rommel Mario Rodriguez Burbano</au><au>Borges, Barbara Do Nascimento</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma</title><date>2018-06-27</date><risdate>2018</risdate><abstract>Abstract Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 samples were obtained. A 675 bp fragment of the S. apella COX-2 promoter region was obtained, and it was 99.2% and 68.2% similar to H. sapiens and S. boliviensis, respectively. Similar to humans, several transcription factors and cis-regulatory element binding sites were identified in the S. apella sequence. MSP revealed that all samples were methylated. However, IHQ results demonstrated positive COX-2 expression in all pre-neoplastic and tumoral samples. The results suggest that the analyzed fragment is not crucial in COX-2 regulation of GC in S. apella.</abstract><pub>SciELO journals</pub><doi>10.6084/m9.figshare.6693476</doi><oa>free_for_read</oa></addata></record> |
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subjects | Biochemistry and Cell Biology not elsewhere classified FOS: Biological sciences Genetics not elsewhere classified |
title | COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma |
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