Transwell data
Migration and invasion assays were performed using Transwell chambers (3422, 24-well insert; 8-μm pore size; Corning Costar, NY, USA) coated with or without Matrigel (356234, BD Biosciences, NJ, USA). 4 × 105 HNSCC cells in DMED medium were placed into the upper chamber, and medium supplementary wit...
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| creator | Pang, Pai Fang, Hui Wu, Hong Wang, Song Liu, Minda Jin, Shan Qi, Zhongzheng Li, Zhenning Wang, Zengxu Liu, Fayu Sun, Changfu |
| description | Migration
and invasion assays were performed using Transwell chambers (3422, 24-well
insert; 8-μm pore size; Corning Costar, NY, USA) coated with or without
Matrigel (356234, BD Biosciences, NJ, USA). 4 × 105 HNSCC cells
in DMED medium were placed into the upper chamber, and medium supplementary
with 10 % FBS was added to the lower chamber. Then PCI-37A cells were incubated
for 24 hours for migration and 48 hours for invasion, and PCI-37A cells were
incubated for 8 hours for migration and 24 hours for invasion. Then the migratory
or invasive cells were fixed by 4% paraformaldehyde, stained with 0.1% crystal
violet, and counted in three random fields under 200x magnification. Transwell migration assay and Martrigel invasion assay
showed that an ectopic increased level of miR-92b could raise the migration and
invasion ability of PCI-37A cells, while a reduced level of miR-92b led to a
significant decrease in the number of migrating or invasive cells.After
knocked down with SP1 expression, PCI-37A/B cells showed a significant decrease
in migration and invasion. |
| doi_str_mv | 10.6084/m9.figshare.6489473 |
| format | Dataset |
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and invasion assays were performed using Transwell chambers (3422, 24-well
insert; 8-μm pore size; Corning Costar, NY, USA) coated with or without
Matrigel (356234, BD Biosciences, NJ, USA). 4 × 105 HNSCC cells
in DMED medium were placed into the upper chamber, and medium supplementary
with 10 % FBS was added to the lower chamber. Then PCI-37A cells were incubated
for 24 hours for migration and 48 hours for invasion, and PCI-37A cells were
incubated for 8 hours for migration and 24 hours for invasion. Then the migratory
or invasive cells were fixed by 4% paraformaldehyde, stained with 0.1% crystal
violet, and counted in three random fields under 200x magnification. Transwell migration assay and Martrigel invasion assay
showed that an ectopic increased level of miR-92b could raise the migration and
invasion ability of PCI-37A cells, while a reduced level of miR-92b led to a
significant decrease in the number of migrating or invasive cells.After
knocked down with SP1 expression, PCI-37A/B cells showed a significant decrease
in migration and invasion.</description><identifier>DOI: 10.6084/m9.figshare.6489473</identifier><language>eng</language><publisher>figshare</publisher><subject>Biochemistry and cell biology not elsewhere classified ; Oncology and carcinogenesis not elsewhere classified</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1894</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.6489473$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Pang, Pai</creatorcontrib><creatorcontrib>Fang, Hui</creatorcontrib><creatorcontrib>Wu, Hong</creatorcontrib><creatorcontrib>Wang, Song</creatorcontrib><creatorcontrib>Liu, Minda</creatorcontrib><creatorcontrib>Jin, Shan</creatorcontrib><creatorcontrib>Qi, Zhongzheng</creatorcontrib><creatorcontrib>Li, Zhenning</creatorcontrib><creatorcontrib>Wang, Zengxu</creatorcontrib><creatorcontrib>Liu, Fayu</creatorcontrib><creatorcontrib>Sun, Changfu</creatorcontrib><title>Transwell data</title><description>Migration
and invasion assays were performed using Transwell chambers (3422, 24-well
insert; 8-μm pore size; Corning Costar, NY, USA) coated with or without
Matrigel (356234, BD Biosciences, NJ, USA). 4 × 105 HNSCC cells
in DMED medium were placed into the upper chamber, and medium supplementary
with 10 % FBS was added to the lower chamber. Then PCI-37A cells were incubated
for 24 hours for migration and 48 hours for invasion, and PCI-37A cells were
incubated for 8 hours for migration and 24 hours for invasion. Then the migratory
or invasive cells were fixed by 4% paraformaldehyde, stained with 0.1% crystal
violet, and counted in three random fields under 200x magnification. Transwell migration assay and Martrigel invasion assay
showed that an ectopic increased level of miR-92b could raise the migration and
invasion ability of PCI-37A cells, while a reduced level of miR-92b led to a
significant decrease in the number of migrating or invasive cells.After
knocked down with SP1 expression, PCI-37A/B cells showed a significant decrease
in migration and invasion.</description><subject>Biochemistry and cell biology not elsewhere classified</subject><subject>Oncology and carcinogenesis not elsewhere classified</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2019</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNo1zrkKwkAYBOBtLCTqC9j4Aol7H6UELxBs0i9_9tBAIrIJiG-vwVhNMTDzIbQmuJBY821nitjc-jukUEiuDVdsjlZVgkf_Cm278TDAAs0itH1YTpmh6rCvylN-uR7P5e6Se21Y7oMO0TvAUTtO6zpiAEwZUKW0MlIIE6MSPjDphfy2wlEqiY5GGeU48SxD7Dc7frpmCPaZmg7S2xJsR6ztjP1j7YRlH1LFOdg</recordid><startdate>20190208</startdate><enddate>20190208</enddate><creator>Pang, Pai</creator><creator>Fang, Hui</creator><creator>Wu, Hong</creator><creator>Wang, Song</creator><creator>Liu, Minda</creator><creator>Jin, Shan</creator><creator>Qi, Zhongzheng</creator><creator>Li, Zhenning</creator><creator>Wang, Zengxu</creator><creator>Liu, Fayu</creator><creator>Sun, Changfu</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20190208</creationdate><title>Transwell data</title><author>Pang, Pai ; Fang, Hui ; Wu, Hong ; Wang, Song ; Liu, Minda ; Jin, Shan ; Qi, Zhongzheng ; Li, Zhenning ; Wang, Zengxu ; Liu, Fayu ; Sun, Changfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d893-de8efdca0f8c42bbf0aa023a2778796559ff75de36d56bf05c22618f9797c41d3</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biochemistry and cell biology not elsewhere classified</topic><topic>Oncology and carcinogenesis not elsewhere classified</topic><toplevel>online_resources</toplevel><creatorcontrib>Pang, Pai</creatorcontrib><creatorcontrib>Fang, Hui</creatorcontrib><creatorcontrib>Wu, Hong</creatorcontrib><creatorcontrib>Wang, Song</creatorcontrib><creatorcontrib>Liu, Minda</creatorcontrib><creatorcontrib>Jin, Shan</creatorcontrib><creatorcontrib>Qi, Zhongzheng</creatorcontrib><creatorcontrib>Li, Zhenning</creatorcontrib><creatorcontrib>Wang, Zengxu</creatorcontrib><creatorcontrib>Liu, Fayu</creatorcontrib><creatorcontrib>Sun, Changfu</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Pang, Pai</au><au>Fang, Hui</au><au>Wu, Hong</au><au>Wang, Song</au><au>Liu, Minda</au><au>Jin, Shan</au><au>Qi, Zhongzheng</au><au>Li, Zhenning</au><au>Wang, Zengxu</au><au>Liu, Fayu</au><au>Sun, Changfu</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>Transwell data</title><date>2019-02-08</date><risdate>2019</risdate><abstract>Migration
and invasion assays were performed using Transwell chambers (3422, 24-well
insert; 8-μm pore size; Corning Costar, NY, USA) coated with or without
Matrigel (356234, BD Biosciences, NJ, USA). 4 × 105 HNSCC cells
in DMED medium were placed into the upper chamber, and medium supplementary
with 10 % FBS was added to the lower chamber. Then PCI-37A cells were incubated
for 24 hours for migration and 48 hours for invasion, and PCI-37A cells were
incubated for 8 hours for migration and 24 hours for invasion. Then the migratory
or invasive cells were fixed by 4% paraformaldehyde, stained with 0.1% crystal
violet, and counted in three random fields under 200x magnification. Transwell migration assay and Martrigel invasion assay
showed that an ectopic increased level of miR-92b could raise the migration and
invasion ability of PCI-37A cells, while a reduced level of miR-92b led to a
significant decrease in the number of migrating or invasive cells.After
knocked down with SP1 expression, PCI-37A/B cells showed a significant decrease
in migration and invasion.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.6489473</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | DOI: 10.6084/m9.figshare.6489473 |
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| language | eng |
| recordid | cdi_datacite_primary_10_6084_m9_figshare_6489473 |
| source | DataCite |
| subjects | Biochemistry and cell biology not elsewhere classified Oncology and carcinogenesis not elsewhere classified |
| title | Transwell data |
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