Additional file 9: of Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier

Despite longer incubation periods, 13-759 and 14-397 do not show an impact on barrier integrity. Barrier integrity of HIBCPP cells was evaluated via measurement of the transepithelial electrical resistance (TEER) (A) at indicated time points after infection with E-30 Bastianni, 13-311, 13-759, or 14-397. TEER values at the start of the experiment (white bars), after 24 h (light gray) and after 48 h (dark gray) are shown.Data are shown as mean + SD of 2 independent experiments carried out in quadruples (B) Live/dead assay on HIBCPP cells after 48 h of infection with E-30 Bastianni, 13-311, 13-759, and 14-397. Representative images of two independent experiments each performed in triplicates are shown (C) HIBCPP cells were infected with E-30 Bastianni, 13-311, 13-759, and 14-397 for 48 h and ZO1 staining was compared; cell layers were stained for nuclei with DAPI (shown here in blue), VP1 (shown here in green), and ZO-1 (red). For detailed description of image acquisition and preparation, please refer to Fig. 2. Two images per strain showing different grouping of parallel staining are displayed horizontally (column one: only ZO-1; column two: DAPI, VP-1, and ZO-1; E-30 strains are listed vertically. The images shown are representative examples of multiple stainings taken from two independent experiments each performed in duplicates. (TIFF 13334 kb)