Additional file 3: of Agrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study

Figure S3. PCR analysis on a set of nine independent transformants obtained through Agrobacterium to study the deletion pattern long the T-DNA. Chlamydomonas cw15 cells were co-cultivated with Agrobacterium C58C1 strain carrying the pAgroLucR plasmid. Six PCR reactions were performed on extracted DNA with nested pairs of oligonucleotides annealing in the T-DNA from the LB to the RB (Panel A). The results (Panel B) show that there is a gradient of deletions from the LB to the RB. β-tubulin was used as positive control for DNA extraction. M: 1 Kb Plus DNA Ladder (Life Technologies); wt: cw15 strain; P: pAgroLucR; −: negative control. Oligonucleotide sequences are reported in Additional file 7: Table S2. (PPTX 204 kb)