Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluatio...
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| creator | Villela, Anne Drumond Valnês S Rodrigues-Junior Antônio Frederico Michel Pinto Falcão, Virgínia Carla De Almeida Zilpa Adriana Sánchez-Quitian Eichler, Paula Bizarro, Cristiano Valim Luiz Augusto Basso Diógenes Santiago Santos |
| description | Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence. |
| doi_str_mv | 10.6084/m9.figshare.5667355 |
| format | Dataset |
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An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. 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An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. 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An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.</abstract><pub>SciELO journals</pub><doi>10.6084/m9.figshare.5667355</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Medicine Parasitology |
| title | Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages |
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