The RIN: an RNA integrity number for assigning integrity values to RNA measurements-5

Copyright information:Taken from "The RIN: an RNA integrity number for assigning integrity values to RNA measurements"BMC Molecular Biology 2006;7():3-3.Published online 31 Jan 2006PMCID:PMC1413964.real-time-PCR experiment, i.e. the average expression values of 4 housekeeping genes (GAPDH, KYNF, NEFL, 2M). The vertical line is a meaningful threshold value for RIN classification, while the horizontal separates acceptable from unacceptable real-time PCR results, a) The RIN shows a strong correlation (0.52) to the expression value of the house keeping genes. A straightforward separation into and is possible. b) The ribosomal ratio shows a poor correlation (0.24) to the expression value of the house keeping genes.