A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion () and snowdrop lectin shows oral toxicity to target insects-4

Copyright information:Taken from "A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion () and snowdrop lectin shows oral toxicity to target insects"BMC Biotechnology 2006;6():18-18.Published online 16 Mar 2006PMCID:PMC1459149.Copyright © 2006 Trung et al; licensee BioMed Central Ltd.ed by 15 % acrylamide SDS-PAGE gel, electroblotted onto nitrocellulose, and probed with anti-GNA antibodies. Loading is as follows: lanes 1, 3, 5 and 11 are control diet, gut tissue, gut contents and haemolymph respectively; lanes 2, 4, 6, 7 and 12 are ButaIT/GNA diet, gut, gut contents and haemolymph; lane 8 is a GNA standard (10 ng); lanes 9 & 10 are ButaIT/GNA standards of 25 and 50 ng, respectively. Loading, as estimated by Bradford assay, was 40 μg total protein for all gut and haemolymph samples.