Detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS-5
Copyright information:Taken from "detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS"http://www.biomedcentral.com/1472-6750/7/69BMC Biotechnology 2007;7():69-69.Published online 18 Oct 2007PMCID:PMC2203993. probe TP-polyA-id33. (B) Dete...
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creator | Stougaard, Magnus Lohmann, Jakob S Zajac, Magdalena Hamilton-Dutoit, Stephen Koch, Jørn |
description | Copyright information:Taken from "detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS"http://www.biomedcentral.com/1472-6750/7/69BMC Biotechnology 2007;7():69-69.Published online 18 Oct 2007PMCID:PMC2203993. probe TP-polyA-id33. (B) Detection of the SSA4-3'UTR RNA fragment with the probe TP-SSA4end-id16. (C) Combined detection of the HPV16E6a RNA and the SSA4-3'UTR RNA fragment with the probes TP-polyA-id33 (green) and TP-SSA4end-id16 (red). The two probes were co-hybridized, co-amplified, and co-detected with a mixture of Lin16 (red identifier) and Lin33 (green identifier). (D-F) Negative controls as (A) save for the following: (D) No capture oligonucleotide present; (E) No RNA was added; (F) The RNA added lacked the artificial polyA tail (HPV16E6noPA RNA) but otherwise had the same sequence as the HPV16E6a RNA used in (A). A 63× objective was used and scale bar is 50 μm. |
doi_str_mv | 10.6084/m9.figshare.47430 |
format | Image |
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(B) Detection of the SSA4-3'UTR RNA fragment with the probe TP-SSA4end-id16. (C) Combined detection of the HPV16E6a RNA and the SSA4-3'UTR RNA fragment with the probes TP-polyA-id33 (green) and TP-SSA4end-id16 (red). The two probes were co-hybridized, co-amplified, and co-detected with a mixture of Lin16 (red identifier) and Lin33 (green identifier). (D-F) Negative controls as (A) save for the following: (D) No capture oligonucleotide present; (E) No RNA was added; (F) The RNA added lacked the artificial polyA tail (HPV16E6noPA RNA) but otherwise had the same sequence as the HPV16E6a RNA used in (A). A 63× objective was used and scale bar is 50 μm.</description><identifier>DOI: 10.6084/m9.figshare.47430</identifier><language>eng</language><publisher>figshare</publisher><subject>Molecular Biology</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1892</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.47430$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Stougaard, Magnus</creatorcontrib><creatorcontrib>Lohmann, Jakob S</creatorcontrib><creatorcontrib>Zajac, Magdalena</creatorcontrib><creatorcontrib>Hamilton-Dutoit, Stephen</creatorcontrib><creatorcontrib>Koch, Jørn</creatorcontrib><title>Detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS-5</title><description>Copyright information:Taken from "detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS"http://www.biomedcentral.com/1472-6750/7/69BMC Biotechnology 2007;7():69-69.Published online 18 Oct 2007PMCID:PMC2203993. probe TP-polyA-id33. (B) Detection of the SSA4-3'UTR RNA fragment with the probe TP-SSA4end-id16. (C) Combined detection of the HPV16E6a RNA and the SSA4-3'UTR RNA fragment with the probes TP-polyA-id33 (green) and TP-SSA4end-id16 (red). The two probes were co-hybridized, co-amplified, and co-detected with a mixture of Lin16 (red identifier) and Lin33 (green identifier). (D-F) Negative controls as (A) save for the following: (D) No capture oligonucleotide present; (E) No RNA was added; (F) The RNA added lacked the artificial polyA tail (HPV16E6noPA RNA) but otherwise had the same sequence as the HPV16E6a RNA used in (A). A 63× objective was used and scale bar is 50 μm.</description><subject>Molecular Biology</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2011</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNqdjksOgjAURTtxYNQFOHsbACHgh6HxE50Qg8ybZ3lgTWlJKQN2LxjdgKOb3JyTHMaWYeBvgl28qhO_lFX7REt-vI2jYMpeR3IknDQaTAnaaK8xqseCdK_QUQFZuofaKBKdoha6VuoK8s46RXCz5jF8qAtwaCty0FhZD441So2ckFaMXHZN7956ziYlqpYW352x8HzKDxevQIdCOuKjjrbnYcDHXl4n_NfLP73RP84bA8hTqQ</recordid><startdate>20111231</startdate><enddate>20111231</enddate><creator>Stougaard, Magnus</creator><creator>Lohmann, Jakob S</creator><creator>Zajac, Magdalena</creator><creator>Hamilton-Dutoit, Stephen</creator><creator>Koch, Jørn</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20111231</creationdate><title>Detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS-5</title><author>Stougaard, Magnus ; Lohmann, Jakob S ; Zajac, Magdalena ; Hamilton-Dutoit, Stephen ; Koch, Jørn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_6084_m9_figshare_474303</frbrgroupid><rsrctype>images</rsrctype><prefilter>images</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Molecular Biology</topic><toplevel>online_resources</toplevel><creatorcontrib>Stougaard, Magnus</creatorcontrib><creatorcontrib>Lohmann, Jakob S</creatorcontrib><creatorcontrib>Zajac, Magdalena</creatorcontrib><creatorcontrib>Hamilton-Dutoit, Stephen</creatorcontrib><creatorcontrib>Koch, Jørn</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Stougaard, Magnus</au><au>Lohmann, Jakob S</au><au>Zajac, Magdalena</au><au>Hamilton-Dutoit, Stephen</au><au>Koch, Jørn</au><format>book</format><genre>unknown</genre><ristype>GEN</ristype><title>Detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS-5</title><date>2011-12-31</date><risdate>2011</risdate><abstract>Copyright information:Taken from "detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS"http://www.biomedcentral.com/1472-6750/7/69BMC Biotechnology 2007;7():69-69.Published online 18 Oct 2007PMCID:PMC2203993. probe TP-polyA-id33. (B) Detection of the SSA4-3'UTR RNA fragment with the probe TP-SSA4end-id16. (C) Combined detection of the HPV16E6a RNA and the SSA4-3'UTR RNA fragment with the probes TP-polyA-id33 (green) and TP-SSA4end-id16 (red). The two probes were co-hybridized, co-amplified, and co-detected with a mixture of Lin16 (red identifier) and Lin33 (green identifier). (D-F) Negative controls as (A) save for the following: (D) No capture oligonucleotide present; (E) No RNA was added; (F) The RNA added lacked the artificial polyA tail (HPV16E6noPA RNA) but otherwise had the same sequence as the HPV16E6a RNA used in (A). A 63× objective was used and scale bar is 50 μm.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.47430</doi><oa>free_for_read</oa></addata></record> |
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subjects | Molecular Biology |
title | Detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS-5 |
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