Representative Scatchard plot of HNECA saturation binding data

Copyright information:Taken from "Aadenosine receptor activity is reduced in neutrophils from patients with systemic sclerosis"Arthritis Research & Therapy 2004;7(2):R189-R195.Published online 10 Dec 2004PMCID:PMC1065314.Copyright © 2004 Bazzichi et al., licensee BioMed Central Ltd. Co...

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Hauptverfasser:	Bazzichi, Laura, Trincavelli, Letizia, Rossi, Alessandra, Feo, Francesca De, Lucacchini, Antonio, Bombardieri, Stefano, Martini, Claudia
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creator	Bazzichi, Laura Trincavelli, Letizia Rossi, Alessandra Feo, Francesca De Lucacchini, Antonio Bombardieri, Stefano Martini, Claudia
description	Copyright information:Taken from "Aadenosine receptor activity is reduced in neutrophils from patients with systemic sclerosis"Arthritis Research & Therapy 2004;7(2):R189-R195.Published online 10 Dec 2004PMCID:PMC1065314.Copyright © 2004 Bazzichi et al., licensee BioMed Central Ltd. Competition binding experiments were performed, incubating aliquots of neutrophil membranes with 20 nmol/l [H]NECA and different NECA concentrations (50 nmol/l to 2 mmol/l), in the presence of 50 nmol/l CPA and 100 nmol/l SCH. Empty circles indicate neutrophil membranes from healthy volunteers (affinity constant [Kd] = 476 ± 34 nmol/l, maximum number of binding sites [B] = 3696 ± 210 fmol/mg); filled circles indicate neutrophil membranes from systemic sclerosis (SSc) patients overall (Kd = 469 ± 35 nmol/l, B= 1292 ± 98 fmol/mg). Assays were performed in triplicate.
doi_str_mv	10.6084/m9.figshare.4325
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Competition binding experiments were performed, incubating aliquots of neutrophil membranes with 20 nmol/l [H]NECA and different NECA concentrations (50 nmol/l to 2 mmol/l), in the presence of 50 nmol/l CPA and 100 nmol/l SCH. Empty circles indicate neutrophil membranes from healthy volunteers (affinity constant [Kd] = 476 ± 34 nmol/l, maximum number of binding sites [B] = 3696 ± 210 fmol/mg); filled circles indicate neutrophil membranes from systemic sclerosis (SSc) patients overall (Kd = 469 ± 35 nmol/l, B= 1292 ± 98 fmol/mg). 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Competition binding experiments were performed, incubating aliquots of neutrophil membranes with 20 nmol/l [H]NECA and different NECA concentrations (50 nmol/l to 2 mmol/l), in the presence of 50 nmol/l CPA and 100 nmol/l SCH. Empty circles indicate neutrophil membranes from healthy volunteers (affinity constant [Kd] = 476 ± 34 nmol/l, maximum number of binding sites [B] = 3696 ± 210 fmol/mg); filled circles indicate neutrophil membranes from systemic sclerosis (SSc) patients overall (Kd = 469 ± 35 nmol/l, B= 1292 ± 98 fmol/mg). 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Competition binding experiments were performed, incubating aliquots of neutrophil membranes with 20 nmol/l [H]NECA and different NECA concentrations (50 nmol/l to 2 mmol/l), in the presence of 50 nmol/l CPA and 100 nmol/l SCH. Empty circles indicate neutrophil membranes from healthy volunteers (affinity constant [Kd] = 476 ± 34 nmol/l, maximum number of binding sites [B] = 3696 ± 210 fmol/mg); filled circles indicate neutrophil membranes from systemic sclerosis (SSc) patients overall (Kd = 469 ± 35 nmol/l, B= 1292 ± 98 fmol/mg). Assays were performed in triplicate.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.4325</doi><oa>free_for_read</oa></addata></record>
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title	Representative Scatchard plot of HNECA saturation binding data
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