() The components of the pGRFP plasmid vector

() The components of the pGRFP plasmid vector

Copyright information:Taken from "A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting"Nucleic Acids Research 2005;33(5):e49-e49.Published online 14 Mar 2005PMCID:PMC1065264.© The Author 2005. Published by Oxford University Press. All...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Choe, Juno, Haiwei H. Guo, Engh, Ger Van Den
Format: Bild
Sprache:eng
Schlagworte:
Uncategorized
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator Choe, Juno
Haiwei H. Guo
Engh, Ger Van Den
description Copyright information:Taken from "A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting"Nucleic Acids Research 2005;33(5):e49-e49.Published online 14 Mar 2005PMCID:PMC1065264.© The Author 2005. Published by Oxford University Press. All rights reserved The main components are the pUC origin of replication, ampicillin resistance marker and a fused GFP-DsRed gene separated by a linker. The linker region is shown with six amino acid linkers (SGSGSG and GSGSGS) on either side, M13 forward and reverse priming sites, and EcoRV, NotI and SalI sites. () Flow-cytometry configuration for dual-fluorescence quantification and sorting. A 488 nm laser excites the fluorescent proteins in individual suspended in the flow stream. The flow cytometer is configured to trigger either on forward scatter or GFP fluorescence. The fluorescence is split using a 550 nm dichroic long pass beam splitter. The green fluorescence is filtered through a 560 nm short pass filter before detection. The red fluorescence passes through a 590 nm long pass filter before detection.
doi_str_mv 10.6084/m9.figshare.4322
format Image
fullrecord <record><control><sourceid>datacite_PQ8</sourceid><recordid>TN_cdi_datacite_primary_10_6084_m9_figshare_4322</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_6084_m9_figshare_4322</sourcerecordid><originalsourceid>FETCH-datacite_primary_10_6084_m9_figshare_43223</originalsourceid><addsrcrecordid>eNpjYJAwNNAzM7Aw0c-11EvLTC_OSCxK1TMxNjLiZNDV0FQIyUhVSM7PLcjPS80rKVbIT1MoAYoUuAe5BSgU5CQW52amKJSlJpfkF_EwsKYl5hSn8kJpbgYDN9cQZw_dlMSSxOTMktT4gqLM3MSiynhDg3iQjfG5lvEwG-NBNhqToQUAz4I71g</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>image</recordtype></control><display><type>image</type><title>() The components of the pGRFP plasmid vector</title><source>DataCite</source><creator>Choe, Juno ; Haiwei H. Guo ; Engh, Ger Van Den</creator><creatorcontrib>Choe, Juno ; Haiwei H. Guo ; Engh, Ger Van Den</creatorcontrib><description>Copyright information:Taken from "A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting"Nucleic Acids Research 2005;33(5):e49-e49.Published online 14 Mar 2005PMCID:PMC1065264.© The Author 2005. Published by Oxford University Press. All rights reserved The main components are the pUC origin of replication, ampicillin resistance marker and a fused GFP-DsRed gene separated by a linker. The linker region is shown with six amino acid linkers (SGSGSG and GSGSGS) on either side, M13 forward and reverse priming sites, and EcoRV, NotI and SalI sites. () Flow-cytometry configuration for dual-fluorescence quantification and sorting. A 488 nm laser excites the fluorescent proteins in individual suspended in the flow stream. The flow cytometer is configured to trigger either on forward scatter or GFP fluorescence. The fluorescence is split using a 550 nm dichroic long pass beam splitter. The green fluorescence is filtered through a 560 nm short pass filter before detection. The red fluorescence passes through a 590 nm long pass filter before detection.</description><identifier>DOI: 10.6084/m9.figshare.4322</identifier><language>eng</language><publisher>figshare</publisher><subject>Uncategorized</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,1888</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.4322$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Choe, Juno</creatorcontrib><creatorcontrib>Haiwei H. Guo</creatorcontrib><creatorcontrib>Engh, Ger Van Den</creatorcontrib><title>() The components of the pGRFP plasmid vector</title><description>Copyright information:Taken from "A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting"Nucleic Acids Research 2005;33(5):e49-e49.Published online 14 Mar 2005PMCID:PMC1065264.© The Author 2005. Published by Oxford University Press. All rights reserved The main components are the pUC origin of replication, ampicillin resistance marker and a fused GFP-DsRed gene separated by a linker. The linker region is shown with six amino acid linkers (SGSGSG and GSGSGS) on either side, M13 forward and reverse priming sites, and EcoRV, NotI and SalI sites. () Flow-cytometry configuration for dual-fluorescence quantification and sorting. A 488 nm laser excites the fluorescent proteins in individual suspended in the flow stream. The flow cytometer is configured to trigger either on forward scatter or GFP fluorescence. The fluorescence is split using a 550 nm dichroic long pass beam splitter. The green fluorescence is filtered through a 560 nm short pass filter before detection. The red fluorescence passes through a 590 nm long pass filter before detection.</description><subject>Uncategorized</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2011</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNpjYJAwNNAzM7Aw0c-11EvLTC_OSCxK1TMxNjLiZNDV0FQIyUhVSM7PLcjPS80rKVbIT1MoAYoUuAe5BSgU5CQW52amKJSlJpfkF_EwsKYl5hSn8kJpbgYDN9cQZw_dlMSSxOTMktT4gqLM3MSiynhDg3iQjfG5lvEwG-NBNhqToQUAz4I71g</recordid><startdate>20111230</startdate><enddate>20111230</enddate><creator>Choe, Juno</creator><creator>Haiwei H. Guo</creator><creator>Engh, Ger Van Den</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20111230</creationdate><title>() The components of the pGRFP plasmid vector</title><author>Choe, Juno ; Haiwei H. Guo ; Engh, Ger Van Den</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_6084_m9_figshare_43223</frbrgroupid><rsrctype>images</rsrctype><prefilter>images</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Uncategorized</topic><toplevel>online_resources</toplevel><creatorcontrib>Choe, Juno</creatorcontrib><creatorcontrib>Haiwei H. Guo</creatorcontrib><creatorcontrib>Engh, Ger Van Den</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Choe, Juno</au><au>Haiwei H. Guo</au><au>Engh, Ger Van Den</au><format>book</format><genre>unknown</genre><ristype>GEN</ristype><title>() The components of the pGRFP plasmid vector</title><date>2011-12-30</date><risdate>2011</risdate><abstract>Copyright information:Taken from "A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting"Nucleic Acids Research 2005;33(5):e49-e49.Published online 14 Mar 2005PMCID:PMC1065264.© The Author 2005. Published by Oxford University Press. All rights reserved The main components are the pUC origin of replication, ampicillin resistance marker and a fused GFP-DsRed gene separated by a linker. The linker region is shown with six amino acid linkers (SGSGSG and GSGSGS) on either side, M13 forward and reverse priming sites, and EcoRV, NotI and SalI sites. () Flow-cytometry configuration for dual-fluorescence quantification and sorting. A 488 nm laser excites the fluorescent proteins in individual suspended in the flow stream. The flow cytometer is configured to trigger either on forward scatter or GFP fluorescence. The fluorescence is split using a 550 nm dichroic long pass beam splitter. The green fluorescence is filtered through a 560 nm short pass filter before detection. The red fluorescence passes through a 590 nm long pass filter before detection.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.4322</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier DOI: 10.6084/m9.figshare.4322
ispartof
issn
language eng
recordid cdi_datacite_primary_10_6084_m9_figshare_4322
source DataCite
subjects Uncategorized
title () The components of the pGRFP plasmid vector
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T16%3A07%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-datacite_PQ8&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.au=Choe,%20Juno&rft.date=2011-12-30&rft_id=info:doi/10.6084/m9.figshare.4322&rft_dat=%3Cdatacite_PQ8%3E10_6084_m9_figshare_4322%3C/datacite_PQ8%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true