IL mutation in cMycÎ 10 partially restores interaction with Max/Max9 and

Copyright information:Taken from "Viral mutations enhance the Max binding properties of the vMyc b-HLH-LZ domain"Nucleic Acids Research 2005;33(16):5235-5242.Published online 15 Sep 2005PMCID:PMC1216337.© The Author 2005. Published by Oxford University Press. All rights reserved () Schematic representation of cMyc, cMycΔ10 and cMycI>LΔ10 mutants. I>L mutation was introduced into cMycΔ10, which does not bind to Max (). () Complex formation between Max/Max9 and cMyc, cMycΔ10, and cMyc I>LΔ10 co-translated was determined by immunoprecipitation of Max/Max9 using an anti-Myc antibody, and [S]-labelled proteins resolved by SDS–PAGE and visualized by fluorography. () Complex formation between Max/Max9 and Pho4-cMyc, Pho4cMycΔ10 and Pho4-cMycI>LΔ10 was measured by co-transfecting the appropriate plasmids into yeast. β-Galactosidase activity was then determined.