A) Quantitative distribution of G, G, Gand Gin different cell fractions: CM (white bars), BLM (gray bars), liver homogenate (dotted bars), RRC (black bars), CURL (down slashed bars), MVB (up slashed bars) and lysosomes (stripped bars

Copyright information:Taken from "Heterotrimeric G protein subunits are located on rat liver endosomes"BMC Physiology 2004;4():1-1.Published online 7 Jan 2004PMCID:PMC324412.Copyright © 2004 Van Dyke; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Optical density of bands for each cell fraction on a single blot were expressed as a fraction of the optical density of the band for CM on the same blot and bars represent the mean ± SEM of results from "n" different preparations of each cell fraction, as indicated on the figure. Except for Gin BLM, G protein subunits were enriched significantly in CM and BLM compared to homogenate (p < 0.0006). G protein subunits in endosomes and lysosomes were significantly less than in CM or BLM (p < 0.0001 to p = 0.027). In vesicles G, G, and Gwere found in the order RRC > CURL > MVB > lysosomes and Gin the order RRC > CURL, MVB > lysosomes. Many of these differences achieved statistical significance: G: RRC vs MVB, p = 0.027; RRC vs lysosomes, p = 0.007; CURL vs lysosomes, p = 0.049. G: RRC vs CURL, p = 0.05; RRC vs lysosomes, p = 0.022. G: RRC vs lysosomes, p = 0.01. G: RRC vs CURL, p = 0.032; RRC vs MVB, p = 0.002; RRC vs lysosomes, p = 0.0006; CURL vs lysosomes, p = 0.008. B) Representative Western blots illustrating data summarized in (A). G: 15 μg protein/lane; others: 30 μg protein/lane; Hom: homogenate.