Prevention of opening abolishes the dequenching of fluorescence
(A and B) Ionic (A) and fluorescence (B) signals recorded from TMRM attached at A397C in control conditions and in the presence of 10 mM 4-AP during 100-ms voltage clamp pulses from −80 to +60 mV. 10 mM 4-AP prevented channel opening and reduced current amplitude by 66 ± 6% ( = 3). (C) Mean G-V and...
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