Formate hydrogenlyase in the hyperthermophilic archaeon, -4

Gment and the genetic organization of the genes. The main subclones are indicated by arrows. . The products of the RT-PCR experiments performed to prove that the genes are located on a single mRNA. cDNA was made using the fhl8frC1R primer, and the amplified regions is indicated with bold lines. Lane 1,17: GeneRuler™ 1 kb DNA Ladder (Fermentas). Templates: lane 2,5,8,11,14: genomic DNA template, lane 3,6,9,12,15: RT(-) experiment lacking reverse transcriptase, lane 4,7,10,13,16: RT(+) experiment. Primers: lane 2–4: fhlBR2-fhlBN1, lane 5–7: fhl02N-fhl03R, lane 8–10: fhl802N-fhl07R, lane 11–13: fhl05R-fhl09N, lane 14–16: fhl8frC1R-fhl12N.Copyright information:Taken from "Formate hydrogenlyase in the hyperthermophilic archaeon, "http://www.biomedcentral.com/1471-2180/8/88BMC Microbiology 2008;8():88-88.Published online 3 Jun 2008PMCID:PMC2432063.