Additional file 1 of Physiological expression of mutated TAU impaired astrocyte activity and exacerbates β-amyloid pathology in 5xFAD mice

Additional file 1: Figure S1. Scheme for generating the 5xFAD TAU transgenic mouse model. The heterozygous Tau mice were bred with the heterozygous 5xFAD and yielded 25% WT, 25% MAPT, 25% 5xFAD and the new desired 5xFAD TAU mice. PCR products were run in Agarose gel. The arrows show the fragment size of APP, PS1 gene and the MAPTgene. Figure S2. Total τ expressionin WT, Tau, 5xFAD and 5xFAD TAU mice reveals physiological levels of τ in all models. Note the slight negative stain circular regions in layer 4/5in 5xFAD and 5xFAD TAU mice due to extensive amyloid deposits. Figure S3. Pictures of gels from figure 2B. Image processing- The bands shown represent gels from the same experiments. All bands were taken from the same gel. Figure S4. Pictures of gels from figure 8C. Image processing. All bands were taken from the same gel.