Additional file 5 of CPT1A in AgRP neurons is required for sex-dependent regulation of feeding and thirst

Additional file 5: Figure S5. Deletion of Cpt1a in AgRP neurons upregulates Cpt1a expression in the liver. a Representative hematoxylin and eosin (H&E) staining of the livers of male and female mice. Scale bar, 50 µm (magnification 20×). b and d Liver weight of female (b, n = 6–8) and male mice (d, n = 6–8). (c and e) Analysis by qRT-PCR of Cpt1a, Cd36, Ucp2, Hmgcs2, Pepck and G6pc mRNA levels in female (c, n = 8) and male mice (e, n = 9–8). f Representative H&E staining of the pancreas and testes from male mice (upper panel) and the pancreas and ovaries from female mice (lower panel). g Plasma level of 17β-estradiol in female mice (n = 8–8) and h testosterone level in male mice (n = 9–10). Scale bar, 50 µm (magnification 20×). Data are expressed as the mean ± SEM. In b–e, * p