Additional file 1 of CD24+CD44+CD54+EpCAM+ gastric cancer stem cells predict tumor progression and metastasis: clinical and experimental evidence
Additional file 1: Figure S1. Percentage of CD24+CD44+CD54+EpCAM+ cells on different days of culture in AGS cells. Cells from the AGS cell line were cultured in non-adherent conditions, without supplemented media, and then harvested for flow cytometry analysis for different cancer stem cell markers...
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| creator | Gómez-Gallegos, Angel A. Ramírez-Vidal, Lizbeth Becerril-Rico, Jared Pérez-Islas, Elizabeth Hernandez-Peralta, Zuly J. Toledo-Guzmán, Mariel E. García-Carrancá, Alejandro Langley, Elizabeth Hernández-Guerrero, Angélica López-Casillas, Fernando Herrera-Goepfert, Roberto Oñate-Ocaña, Luis F. Ortiz-Sánchez, Elizabeth |
| description | Additional file 1: Figure S1. Percentage of CD24+CD44+CD54+EpCAM+ cells on different days of culture in AGS cells. Cells from the AGS cell line were cultured in non-adherent conditions, without supplemented media, and then harvested for flow cytometry analysis for different cancer stem cell markers on day 0, 1, 3, 5 and 7 of culture. We observed an increase in the CD24+CD44+CD54+EpCAM+ on day 3 compared with other days. Ten thousand cells were acquired by the NXT Attune cytometer. Error bars indicate the ± SD of three independent assays. *P ≤ 0.05. |
| doi_str_mv | 10.6084/m9.figshare.26566118 |
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Percentage of CD24+CD44+CD54+EpCAM+ cells on different days of culture in AGS cells. Cells from the AGS cell line were cultured in non-adherent conditions, without supplemented media, and then harvested for flow cytometry analysis for different cancer stem cell markers on day 0, 1, 3, 5 and 7 of culture. We observed an increase in the CD24+CD44+CD54+EpCAM+ on day 3 compared with other days. Ten thousand cells were acquired by the NXT Attune cytometer. 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Percentage of CD24+CD44+CD54+EpCAM+ cells on different days of culture in AGS cells. Cells from the AGS cell line were cultured in non-adherent conditions, without supplemented media, and then harvested for flow cytometry analysis for different cancer stem cell markers on day 0, 1, 3, 5 and 7 of culture. We observed an increase in the CD24+CD44+CD54+EpCAM+ on day 3 compared with other days. Ten thousand cells were acquired by the NXT Attune cytometer. 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Percentage of CD24+CD44+CD54+EpCAM+ cells on different days of culture in AGS cells. Cells from the AGS cell line were cultured in non-adherent conditions, without supplemented media, and then harvested for flow cytometry analysis for different cancer stem cell markers on day 0, 1, 3, 5 and 7 of culture. We observed an increase in the CD24+CD44+CD54+EpCAM+ on day 3 compared with other days. Ten thousand cells were acquired by the NXT Attune cytometer. Error bars indicate the ± SD of three independent assays. *P ≤ 0.05.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.26566118</doi><orcidid>https://orcid.org/0000-0001-9855-9491</orcidid><oa>free_for_read</oa></addata></record> |
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| title | Additional file 1 of CD24+CD44+CD54+EpCAM+ gastric cancer stem cells predict tumor progression and metastasis: clinical and experimental evidence |
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