MiR ISH analysis in the mouse retina

Copyright information:Taken from "Altered retinal microRNA expression profile in a mouse model of retinitis pigmentosa"http://genomebiology.com/2007/8/11/R248Genome Biology 2007;8(11):R248-R248.Published online 22 Nov 2007PMCID:PMC2258196. Eyes from 1-month-old c57 animals were fixed in 4% paraformaldehyde, and 12 μm cryosections were hybridized with 5'-digoxigenin labeled locked nucleic acid (LNA) microRNA (miR) probes for let-7, miR-181a, and miR-182. A false-colored (magenta) 4',6-diamidine-2-phenylindole-dihydrochloride (DAPI) nuclear staining is overlaid on the miR-182 hybridization (ISH) label (panel d) to indicate the position of the nuclear layers. Scale bar: 25 μm. GCL, ganglion cell layer; INL, inner nuclear layer; IS, photoreceptor inner segments; ONL, outer nuclear layer; OS, photoreceptor outer segments.