Additional file 4 of Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response

Additional file 4. Fig. S4. ZIKV infection does not induce pathological Tau phosphorylation recognized by AT100 and AD2 antibodies in the brain of CC001 and CC071 mice. CC001 and CC071 mice (5-6 week old) were necropsied at day 6 following IC inoculation of either PBS or 105 FFU of ZIKV. No induction of pTau recognized by AT100 and AD2 antibodies was observed in the brain of ZIKV- as compared to PBS-treated mice as determined by immunofluorescence and confocal microscopy with nuclear DNA labeled with DAPI (blue), pTau labeled with (a) the AT100 or (b) AD2 antibodies (green). Single confocal sections (z projection) and the corresponding merge images are shown. Scale bars= 50 µm.