Additional file 5 of PAQR8 promotes breast cancer recurrence and confers resistance to multiple therapies

Additional file 5: Paqr8 does not affect rates of apoptosis or cell proliferation in the presence of Her2 expression in vivo or in vitro. nu/nu mice harboring 5x5mm primary tumors from orthotopically injected Her2-dependent Paqr8-OE (a, c) or Paqr8-KO (b, d) primary mouse tumor cells were injected with 50mg/kg EdU (i.p.) 2h prior to sacrifice. Primary tumors were harvested, sectioned, and stained by immunofluorescence for cleaved caspase-3 (cc3), EdU, and Ki67. a-d Percentage of eGFP+ cells that were cc3+, EdU+, or Ki67+ as indicated. Paqr8-OE (e, g) or Paqr8-KO (f, h) Her2-dependent primary cells were cultured in the presence of 10% serum with doxycycline (Her2 ON) and incubated with 10mM EdU for 2h prior to fixation, permeabilization, and staining for cc3, EdU, and Ki67. e-h Percentage of eGFP+ that were cc3+, EdU+, or Ki67+, as indicated.