Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
ABSTRACT Background: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. Methods: A specific region of DNA sequences...
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| creator | Fernandes, Blenda Santrer, Emanoelle Fernandes Rutren La Figueiredo, Sônia Maria de Rocha-Silva, Fabiana Assunção, Cláudia Barbosa Abreu, Amanda Gabrielle Santiago, Iara Furtado Johann, Susana Caligiorne, Rachel Basques |
| description | ABSTRACT Background: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. Methods: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. Results: Three specific primers with 100% specificity for the Sporothrix genus were generated. Conclusions: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis. |
| doi_str_mv | 10.6084/m9.figshare.22225547 |
| format | Dataset |
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This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. Methods: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. Results: Three specific primers with 100% specificity for the Sporothrix genus were generated. 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This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. Methods: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. Results: Three specific primers with 100% specificity for the Sporothrix genus were generated. Conclusions: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.</abstract><pub>SciELO journals</pub><doi>10.6084/m9.figshare.22225547</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Medicine |
| title | Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus |
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