Root canal microbiota as an augmented reservoir of antimicrobial resistance genes in type 2 diabetes mellitus patients
Abstract Antimicrobial resistance is a global public health problem. Root canal microbiota associated with apical periodontitis represents a well-known reservoir of antimicrobial resistance genes (ARGs). However, the effect of type 2 diabetes mellitus (T2DM) in this reservoir is unknown. This study...
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| creator | VÁZQUEZ-RAMOS, Víctor Rafael PÉREZ-SERRANO, Rosa Martha GARCÍA-SOLÍS, Pablo SOLÍS-SAINZ, Juan Carlos ESPINOSA-CRISTÓBAL, León Francisco CASTRO-RUÍZ, Jesús Eduardo DOMÍNGUEZ-PÉREZ, Rubén Abraham |
| description | Abstract Antimicrobial resistance is a global public health problem. Root canal microbiota associated with apical periodontitis represents a well-known reservoir of antimicrobial resistance genes (ARGs). However, the effect of type 2 diabetes mellitus (T2DM) in this reservoir is unknown. This study aimed to establish if root canal microbiota associated with apical periodontitis in T2DM patients is an augmented reservoir by identifying the prevalence of nine common ARGs and comparing it with the prevalence in nondiabetic patients. Methodology This cross-sectional study included two groups: A T2DM group conformed of 20 patients with at least ten years of living with T2DM and a control group of 30 nondiabetic participants. Premolar or molar teeth with pulp necrosis and apical periodontitis were included. A sample was collected from each root canal before endodontic treatment. DNA was extracted, and ARGs were identified by polymerase chain reaction. Results tetW and tetM genes were the most frequent (93.3 and 91.6%, respectively), while ermA was the least frequent (8.3%) in the total population. The distribution of the ARGs was similar in both groups, but a significant difference (p |
| doi_str_mv | 10.6084/m9.figshare.21981190 |
| format | Dataset |
| fullrecord | <record><control><sourceid>datacite_PQ8</sourceid><recordid>TN_cdi_datacite_primary_10_6084_m9_figshare_21981190</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_6084_m9_figshare_21981190</sourcerecordid><originalsourceid>FETCH-LOGICAL-d910-9be7eef91b96b0718e938d9eb249fe850a9f5d22769661b010e4cd6c2fd00d013</originalsourceid><addsrcrecordid>eNo1kMtqwzAQRb3poqT9gy7mB-xKjmN7liX0BYFCyV6MrJErsCUjKYH8fV2arC7c1-IUxZMUVSv65nnGyrox_VDkqpbYS4nivjh_h5BhIE8TzG6IQbuQCSgBeaDTOLPPbCBy4ngOLkKwa5Ldtbuu1silTH5gGNlzAuchXxaGGowjzXm1Zp4ml08JFspufUwPxZ2lKfHjVTfF8e31uP8oD1_vn_uXQ2lQihI1d8wWpcZWi072jNveIOu6Qcv9ThDananrrsW2lVpIwc1g2qG2Rggj5HZTNP-3hjINLrNaopspXpQU6o-KmlHdqKgble0vJgxhCg</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>dataset</recordtype></control><display><type>dataset</type><title>Root canal microbiota as an augmented reservoir of antimicrobial resistance genes in type 2 diabetes mellitus patients</title><source>DataCite</source><creator>VÁZQUEZ-RAMOS, Víctor Rafael ; PÉREZ-SERRANO, Rosa Martha ; GARCÍA-SOLÍS, Pablo ; SOLÍS-SAINZ, Juan Carlos ; ESPINOSA-CRISTÓBAL, León Francisco ; CASTRO-RUÍZ, Jesús Eduardo ; DOMÍNGUEZ-PÉREZ, Rubén Abraham</creator><creatorcontrib>VÁZQUEZ-RAMOS, Víctor Rafael ; PÉREZ-SERRANO, Rosa Martha ; GARCÍA-SOLÍS, Pablo ; SOLÍS-SAINZ, Juan Carlos ; ESPINOSA-CRISTÓBAL, León Francisco ; CASTRO-RUÍZ, Jesús Eduardo ; DOMÍNGUEZ-PÉREZ, Rubén Abraham</creatorcontrib><description>Abstract Antimicrobial resistance is a global public health problem. Root canal microbiota associated with apical periodontitis represents a well-known reservoir of antimicrobial resistance genes (ARGs). However, the effect of type 2 diabetes mellitus (T2DM) in this reservoir is unknown. This study aimed to establish if root canal microbiota associated with apical periodontitis in T2DM patients is an augmented reservoir by identifying the prevalence of nine common ARGs and comparing it with the prevalence in nondiabetic patients. Methodology This cross-sectional study included two groups: A T2DM group conformed of 20 patients with at least ten years of living with T2DM and a control group of 30 nondiabetic participants. Premolar or molar teeth with pulp necrosis and apical periodontitis were included. A sample was collected from each root canal before endodontic treatment. DNA was extracted, and ARGs were identified by polymerase chain reaction. Results tetW and tetM genes were the most frequent (93.3 and 91.6%, respectively), while ermA was the least frequent (8.3%) in the total population. The distribution of the ARGs was similar in both groups, but a significant difference (p<0.005) was present in ermB, ermC, cfxA, and tetQ genes, being more frequent in the T2DM group. A total of eighty percent of the T2DM patients presented a minimum of four ARGs, while 76.6% of the control group presented a maximum of three. Conclusions Root canal microbiota associated with apical periodontitis in T2DM patients carries more ARGs. Therefore, this pathological niche could be considered an augmented reservoir.</description><identifier>DOI: 10.6084/m9.figshare.21981190</identifier><language>eng</language><publisher>SciELO journals</publisher><subject>Dentistry not elsewhere classified ; FOS: Clinical medicine</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,1888</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.21981190$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>VÁZQUEZ-RAMOS, Víctor Rafael</creatorcontrib><creatorcontrib>PÉREZ-SERRANO, Rosa Martha</creatorcontrib><creatorcontrib>GARCÍA-SOLÍS, Pablo</creatorcontrib><creatorcontrib>SOLÍS-SAINZ, Juan Carlos</creatorcontrib><creatorcontrib>ESPINOSA-CRISTÓBAL, León Francisco</creatorcontrib><creatorcontrib>CASTRO-RUÍZ, Jesús Eduardo</creatorcontrib><creatorcontrib>DOMÍNGUEZ-PÉREZ, Rubén Abraham</creatorcontrib><title>Root canal microbiota as an augmented reservoir of antimicrobial resistance genes in type 2 diabetes mellitus patients</title><description>Abstract Antimicrobial resistance is a global public health problem. Root canal microbiota associated with apical periodontitis represents a well-known reservoir of antimicrobial resistance genes (ARGs). However, the effect of type 2 diabetes mellitus (T2DM) in this reservoir is unknown. This study aimed to establish if root canal microbiota associated with apical periodontitis in T2DM patients is an augmented reservoir by identifying the prevalence of nine common ARGs and comparing it with the prevalence in nondiabetic patients. Methodology This cross-sectional study included two groups: A T2DM group conformed of 20 patients with at least ten years of living with T2DM and a control group of 30 nondiabetic participants. Premolar or molar teeth with pulp necrosis and apical periodontitis were included. A sample was collected from each root canal before endodontic treatment. DNA was extracted, and ARGs were identified by polymerase chain reaction. Results tetW and tetM genes were the most frequent (93.3 and 91.6%, respectively), while ermA was the least frequent (8.3%) in the total population. The distribution of the ARGs was similar in both groups, but a significant difference (p<0.005) was present in ermB, ermC, cfxA, and tetQ genes, being more frequent in the T2DM group. A total of eighty percent of the T2DM patients presented a minimum of four ARGs, while 76.6% of the control group presented a maximum of three. Conclusions Root canal microbiota associated with apical periodontitis in T2DM patients carries more ARGs. Therefore, this pathological niche could be considered an augmented reservoir.</description><subject>Dentistry not elsewhere classified</subject><subject>FOS: Clinical medicine</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2023</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNo1kMtqwzAQRb3poqT9gy7mB-xKjmN7liX0BYFCyV6MrJErsCUjKYH8fV2arC7c1-IUxZMUVSv65nnGyrox_VDkqpbYS4nivjh_h5BhIE8TzG6IQbuQCSgBeaDTOLPPbCBy4ngOLkKwa5Ldtbuu1silTH5gGNlzAuchXxaGGowjzXm1Zp4ml08JFspufUwPxZ2lKfHjVTfF8e31uP8oD1_vn_uXQ2lQihI1d8wWpcZWi072jNveIOu6Qcv9ThDananrrsW2lVpIwc1g2qG2Rggj5HZTNP-3hjINLrNaopspXpQU6o-KmlHdqKgble0vJgxhCg</recordid><startdate>20230131</startdate><enddate>20230131</enddate><creator>VÁZQUEZ-RAMOS, Víctor Rafael</creator><creator>PÉREZ-SERRANO, Rosa Martha</creator><creator>GARCÍA-SOLÍS, Pablo</creator><creator>SOLÍS-SAINZ, Juan Carlos</creator><creator>ESPINOSA-CRISTÓBAL, León Francisco</creator><creator>CASTRO-RUÍZ, Jesús Eduardo</creator><creator>DOMÍNGUEZ-PÉREZ, Rubén Abraham</creator><general>SciELO journals</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20230131</creationdate><title>Root canal microbiota as an augmented reservoir of antimicrobial resistance genes in type 2 diabetes mellitus patients</title><author>VÁZQUEZ-RAMOS, Víctor Rafael ; PÉREZ-SERRANO, Rosa Martha ; GARCÍA-SOLÍS, Pablo ; SOLÍS-SAINZ, Juan Carlos ; ESPINOSA-CRISTÓBAL, León Francisco ; CASTRO-RUÍZ, Jesús Eduardo ; DOMÍNGUEZ-PÉREZ, Rubén Abraham</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d910-9be7eef91b96b0718e938d9eb249fe850a9f5d22769661b010e4cd6c2fd00d013</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Dentistry not elsewhere classified</topic><topic>FOS: Clinical medicine</topic><toplevel>online_resources</toplevel><creatorcontrib>VÁZQUEZ-RAMOS, Víctor Rafael</creatorcontrib><creatorcontrib>PÉREZ-SERRANO, Rosa Martha</creatorcontrib><creatorcontrib>GARCÍA-SOLÍS, Pablo</creatorcontrib><creatorcontrib>SOLÍS-SAINZ, Juan Carlos</creatorcontrib><creatorcontrib>ESPINOSA-CRISTÓBAL, León Francisco</creatorcontrib><creatorcontrib>CASTRO-RUÍZ, Jesús Eduardo</creatorcontrib><creatorcontrib>DOMÍNGUEZ-PÉREZ, Rubén Abraham</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>VÁZQUEZ-RAMOS, Víctor Rafael</au><au>PÉREZ-SERRANO, Rosa Martha</au><au>GARCÍA-SOLÍS, Pablo</au><au>SOLÍS-SAINZ, Juan Carlos</au><au>ESPINOSA-CRISTÓBAL, León Francisco</au><au>CASTRO-RUÍZ, Jesús Eduardo</au><au>DOMÍNGUEZ-PÉREZ, Rubén Abraham</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>Root canal microbiota as an augmented reservoir of antimicrobial resistance genes in type 2 diabetes mellitus patients</title><date>2023-01-31</date><risdate>2023</risdate><abstract>Abstract Antimicrobial resistance is a global public health problem. Root canal microbiota associated with apical periodontitis represents a well-known reservoir of antimicrobial resistance genes (ARGs). However, the effect of type 2 diabetes mellitus (T2DM) in this reservoir is unknown. This study aimed to establish if root canal microbiota associated with apical periodontitis in T2DM patients is an augmented reservoir by identifying the prevalence of nine common ARGs and comparing it with the prevalence in nondiabetic patients. Methodology This cross-sectional study included two groups: A T2DM group conformed of 20 patients with at least ten years of living with T2DM and a control group of 30 nondiabetic participants. Premolar or molar teeth with pulp necrosis and apical periodontitis were included. A sample was collected from each root canal before endodontic treatment. DNA was extracted, and ARGs were identified by polymerase chain reaction. Results tetW and tetM genes were the most frequent (93.3 and 91.6%, respectively), while ermA was the least frequent (8.3%) in the total population. The distribution of the ARGs was similar in both groups, but a significant difference (p<0.005) was present in ermB, ermC, cfxA, and tetQ genes, being more frequent in the T2DM group. A total of eighty percent of the T2DM patients presented a minimum of four ARGs, while 76.6% of the control group presented a maximum of three. Conclusions Root canal microbiota associated with apical periodontitis in T2DM patients carries more ARGs. Therefore, this pathological niche could be considered an augmented reservoir.</abstract><pub>SciELO journals</pub><doi>10.6084/m9.figshare.21981190</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Dentistry not elsewhere classified FOS: Clinical medicine |
| title | Root canal microbiota as an augmented reservoir of antimicrobial resistance genes in type 2 diabetes mellitus patients |
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