Structural and functional analysis of the gene promoter-6

Copyright information:Taken from "Structural and functional analysis of the gene promoter"http://www.biomedcentral.com/1471-2199/8/82BMC Molecular Biology 2007;8():82-82.Published online 20 Sep 2007PMCID:PMC2064931.putative cis-acting consensus sequences (boxes). Arrows indicate the transcription initiation sites. The 683 bp DNA fragment from the 5'-flanking region of was cloned upstream of the reporter gene into the pBSCAT-ACT vector. trophozoites were transfected with this construction and incubated at 37°C for 48 h. Then, trophozoites were incubated for 2 and 4 h more at 37°C or at 42°C and CAT activities were determined. Activities are relative to that obtained with the promoter gene at 37°C, used as positive control. Each bar corresponds to the average of CAT activities ± S.D. representative of three independent experiments performed by duplicate. Real-time RT-PCR assays using RNA from trophozoites grown at 37°C (A) or after 4 h of heat shock treatment (A-HS) and specific primers for . Amplification of the 18S rRNA was used as normalizer.