Additional file 3 of Synergistic effects of ISL1 and KDM6B on non-alcoholic fatty liver disease through the regulation of SNAI1

Additional file 3: Fig. S3 Experimental images of Oil red O staining and immunohistochemistry. A, Oil red O staining was applied to detect liver lipid accumulation in mice exposed to high-fat diet; B, Immunohistochemistry was used to detect the expression of ISL1 in liver tissues of mice exposed to high-fat diet; C, Oil red O staining was performed to detect lipid accumulation; D, Oil red O staining was applied to detect the effect of overexpressed ISL1 on liver lipid accumulation in mouse model; E, Oil red O staining was performed to detect the effect of ISL1 on lipid accumulation; F, Oil red O staining shows steatosis; G, Oil red O staining was applied to detect the effect of overexpressing KDM6B and SNAI1 on liver lipid accumulation in mouse model; H, Oil red O staining was performed to detect the effect of KDM6B and SNAI1 on lipid accumulation; I, Oil red O staining was applied to detect the effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on liver lipid accumulation in mouse model; J, Oil red O staining was performed to detect the effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on lipid accumulation. n = 8 mice in each group.