Additional file 2 of Interplay between nitric oxide and gonadotrophin-releasing hormone in the neuromodulation of the corpus luteum during late pregnancy in the rat

Additional file 2: Supplementary Figure 2. Effect of stimulation of the CG with 100 μM L-NAME on the concentration of nitrites in the incubation medium of the left ovary, during the first proestrus in the rat. For this, the CG-SON-Ovary system was extracted from 37-day-old Holtzman rats and immediately placed in a cuvette with two compartments, one for the CG and the other for the ovary, both joined by the SON. The incubation medium was 1 ml of Krebs-Ringer bicarbonate buffer, pH 7.4, with 0.1 mg/ml dextrose and 0.1 mg/ml BSA at 37 °C in a saturated atmosphere of 95% O2 and 5% CO2. The results show that the addition of this nitric oxide synthase inhibitor in the ganglion compartment decreases the nitrite concentration in the ovarian incubation medium, throughout 180 min of incubation. The values represent the mean ± SEM of 6 animals per experimental group. Two-way ANOVA followed by Bonferroni’s multiple comparison tests: (*) P