Additional file 2 of Biologic TNF-α inhibitors reduce microgliosis, neuronal loss, and tau phosphorylation in a transgenic mouse model of tauopathy
Additional file 2: Fig. S1 High-affinity binding of the TfRMAb-TNFR to the TfR (A) and TNF-α (B). iPSC-derived brain endothelial cells (ihBMECs) cultured in Transwell inserts were treated with 1 µg/mL TNF-α and 7 µg/mL TfRMAb-TNFR for up to 3 days. TNF-α reduced TEER (C) and increased sodium fluores...
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| creator | Ou, Weijun Yang, Joshua Simanauskaite, Juste Choi, Matthew Castellanos, Demi M. Chang, Rudy Sun, Jiahong Jagadeesan, Nataraj Parfitt, Karen D. Cribbs, David H. Sumbria, Rachita K. |
| description | Additional file 2: Fig. S1 High-affinity binding of the TfRMAb-TNFR to the TfR (A) and TNF-α (B). iPSC-derived brain endothelial cells (ihBMECs) cultured in Transwell inserts were treated with 1 µg/mL TNF-α and 7 µg/mL TfRMAb-TNFR for up to 3 days. TNF-α reduced TEER (C) and increased sodium fluorescein passage across the brain endothelial monolayer (D). These effects were normalized by TfRMAb-TNFR treatment. All the experiments were repeated three times independently and data were expressed as mean ± SEM. Two-way repeated-measures ANOVA or one-way ANOVA with Holm Sidak’s post hoc test was used in C and D, respectively. *p |
| doi_str_mv | 10.6084/m9.figshare.17711039 |
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TNF-α reduced TEER (C) and increased sodium fluorescein passage across the brain endothelial monolayer (D). These effects were normalized by TfRMAb-TNFR treatment. All the experiments were repeated three times independently and data were expressed as mean ± SEM. Two-way repeated-measures ANOVA or one-way ANOVA with Holm Sidak’s post hoc test was used in C and D, respectively. *p<0.05, ***p<0.001, ***p<0.0001. 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| title | Additional file 2 of Biologic TNF-α inhibitors reduce microgliosis, neuronal loss, and tau phosphorylation in a transgenic mouse model of tauopathy |
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