scRNAseq of cancer associated fibroblasts (CAFs) in Merkel cell carcinoma (MCC)

The "CAF_raw_expression_counts.txt"-file contains raw expression counts of MCC associated fibroblast measured by 10XGenomics 5' scRNAseq. The data set comprises cells of fibroblasts from 3 different samples. The corresponding meta data file is called "CAF_cell_metadata.csv" and provides cell annotations. The corresponding data set containing normalized counts is called "CAF_normalized_expression_counts.txt". For cell preparation, tumor cells were dissociated into single-cell suspensions and barcoded using the 10x Genomics Chromium v2.0 platform (10x Genomics, Leiden, Netherland). Library preparation was performed according to the 10x Genomics protocol, and the library was sequenced on an Illumina HiSeq 4000 platform (Illumina, Eindhoven, Netherland). Cell Ranger Single-Cell Software Suite version 2.1.1 (http://10xgenomics.com/) was used to align cDNA reads to the hg19 human reference genome. Cell types were annotated using marker genes and fibroblasts were extracted.