Additional file 3 of JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells

Additional file 3: Figure S3. Supplemental data for Fig. 7. Neutrophils were stimulated with GM-CSF for 24 h in the presence or absence of the pretreatment JAKi (tofacitinib, baricitinib, upadacitinib) for 1 h. Cellular lysates were analyzed by Western using anti-NLRP3 or anti-β-actin antibodies. Three experiments were performed using different neutrophils and a representative result is shown.