Additional file 2 of JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells

Additional file 2: Figure S2. Supplemental data for Fig. 4b. THP-1 cells were pretreated with JAKi (tofacitinib, baricitinib, upadacitinib) at the indicated (25, 100 nM) for 1 h and then stimulated with GM-CSF (20 ng/ml) for 20 min. Phosphorylation of STAT5 was determined by Western blotting using p...

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Hauptverfasser:	Fujita, Yuya, Matsuoka, Naoki, Temmoku, Jumpei, Furuya-Yashiro, Makiko, Asano, Tomoyuki, Sato, Shuzo, Matsumoto, Haruki, Watanabe, Hiroshi, Kozuru, Hideko, Yatsuhashi, Hiroshi, Kawakami, Atsushi, Migita, Kiyoshi
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Sprache:	eng
Schlagworte:	FOS: Clinical medicine Immunology
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creator	Fujita, Yuya Matsuoka, Naoki Temmoku, Jumpei Furuya-Yashiro, Makiko Asano, Tomoyuki Sato, Shuzo Matsumoto, Haruki Watanabe, Hiroshi Kozuru, Hideko Yatsuhashi, Hiroshi Kawakami, Atsushi Migita, Kiyoshi
description	Additional file 2: Figure S2. Supplemental data for Fig. 4b. THP-1 cells were pretreated with JAKi (tofacitinib, baricitinib, upadacitinib) at the indicated (25, 100 nM) for 1 h and then stimulated with GM-CSF (20 ng/ml) for 20 min. Phosphorylation of STAT5 was determined by Western blotting using phospho-specific antibodies against STAT5. Three experiments were performed and a representative result is shown.
doi_str_mv	10.6084/m9.figshare.12486854
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Supplemental data for Fig. 4b. THP-1 cells were pretreated with JAKi (tofacitinib, baricitinib, upadacitinib) at the indicated (25, 100 nM) for 1 h and then stimulated with GM-CSF (20 ng/ml) for 20 min. Phosphorylation of STAT5 was determined by Western blotting using phospho-specific antibodies against STAT5. 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Supplemental data for Fig. 4b. THP-1 cells were pretreated with JAKi (tofacitinib, baricitinib, upadacitinib) at the indicated (25, 100 nM) for 1 h and then stimulated with GM-CSF (20 ng/ml) for 20 min. Phosphorylation of STAT5 was determined by Western blotting using phospho-specific antibodies against STAT5. Three experiments were performed and a representative result is shown.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.12486854</doi><oa>free_for_read</oa></addata></record>
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subjects	FOS: Clinical medicine Immunology
title	Additional file 2 of JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells
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