CIL:13577, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library
Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of Helix Pomatia Lectin (HPL) (green) from the Golgi. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to termin...
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| creator | Gill, David J. Chia, Joanne Senewiratne, Jamie Bard, Frederic |
| description | Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of Helix Pomatia Lectin (HPL) (green) from the Golgi. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HPL indicates distribution of GalNac-Ts. Inactive Src is highly enriched at the Golgi. Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100) prior to staining with Hoechst (blue) and Alexa 647-conjugated-HPL. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with either a 60x objective (U Plan Super Apochromatic, UPLSAPO; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 7F in J Cell Biol. 189: 843-858. 2010. Images in Fig 7 include CIL#s 13571, 13572, 13573, 13574, 13575, 13576, 13577. |
| doi_str_mv | 10.6075/j0mc8xqf |
| format | Dataset |
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In Cell Image Library</title><source>DataCite</source><creator>Gill, David J. ; Chia, Joanne ; Senewiratne, Jamie ; Bard, Frederic</creator><creatorcontrib>Gill, David J. ; Chia, Joanne ; Senewiratne, Jamie ; Bard, Frederic</creatorcontrib><description>Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of Helix Pomatia Lectin (HPL) (green) from the Golgi. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HPL indicates distribution of GalNac-Ts. Inactive Src is highly enriched at the Golgi. Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100) prior to staining with Hoechst (blue) and Alexa 647-conjugated-HPL. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with either a 60x objective (U Plan Super Apochromatic, UPLSAPO; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 7F in J Cell Biol. 189: 843-858. 2010. Images in Fig 7 include CIL#s 13571, 13572, 13573, 13574, 13575, 13576, 13577.</description><identifier>DOI: 10.6075/j0mc8xqf</identifier><language>eng</language><publisher>UC San Diego Library Digital Collections</publisher><subject>cervical carcinoma ; COPI coating of Golgi vesicle ; epithelial cell ; Golgi organization ; Golgi stack ; GTPase activity ; HeLa ; Homo sapiens ; integral to membrane ; nucleus ; protein binding ; retrograde vesicle-mediated transport, Golgi to ER ; small GTPase mediated signal transduction</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,1888</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6075/j0mc8xqf$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Gill, David J.</creatorcontrib><creatorcontrib>Chia, Joanne</creatorcontrib><creatorcontrib>Senewiratne, Jamie</creatorcontrib><creatorcontrib>Bard, Frederic</creatorcontrib><title>CIL:13577, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library</title><description>Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of Helix Pomatia Lectin (HPL) (green) from the Golgi. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HPL indicates distribution of GalNac-Ts. Inactive Src is highly enriched at the Golgi. Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100) prior to staining with Hoechst (blue) and Alexa 647-conjugated-HPL. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with either a 60x objective (U Plan Super Apochromatic, UPLSAPO; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 7F in J Cell Biol. 189: 843-858. 2010. Images in Fig 7 include CIL#s 13571, 13572, 13573, 13574, 13575, 13576, 13577.</description><subject>cervical carcinoma</subject><subject>COPI coating of Golgi vesicle</subject><subject>epithelial cell</subject><subject>Golgi organization</subject><subject>Golgi stack</subject><subject>GTPase activity</subject><subject>HeLa</subject><subject>Homo sapiens</subject><subject>integral to membrane</subject><subject>nucleus</subject><subject>protein binding</subject><subject>retrograde vesicle-mediated transport, Golgi to ER</subject><subject>small GTPase mediated signal transduction</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2021</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNpjYBAwNNAzMzA31c8yyE22qChM42QIdvb0sTI0NjU311HwyM_NVyhOLMhMzSvWUUgtyCzJSM3JTMxRSE7NydEBkkVlmckgbmJRcmZefm6inoJnnoIzUFLBMzcxPVXBJzOpKLGokoeBNS0xpziVF0pzM2i4uYY4e-imJJYkJmeWpMYXFGXmAhXGGxrEg9wTD3OPMQlKAZbWQR4</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Gill, David J.</creator><creator>Chia, Joanne</creator><creator>Senewiratne, Jamie</creator><creator>Bard, Frederic</creator><general>UC San Diego Library Digital Collections</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>2021</creationdate><title>CIL:13577, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library</title><author>Gill, David J. ; Chia, Joanne ; Senewiratne, Jamie ; Bard, Frederic</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_6075_j0mc8xqf3</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2021</creationdate><topic>cervical carcinoma</topic><topic>COPI coating of Golgi vesicle</topic><topic>epithelial cell</topic><topic>Golgi organization</topic><topic>Golgi stack</topic><topic>GTPase activity</topic><topic>HeLa</topic><topic>Homo sapiens</topic><topic>integral to membrane</topic><topic>nucleus</topic><topic>protein binding</topic><topic>retrograde vesicle-mediated transport, Golgi to ER</topic><topic>small GTPase mediated signal transduction</topic><toplevel>online_resources</toplevel><creatorcontrib>Gill, David J.</creatorcontrib><creatorcontrib>Chia, Joanne</creatorcontrib><creatorcontrib>Senewiratne, Jamie</creatorcontrib><creatorcontrib>Bard, Frederic</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Gill, David J.</au><au>Chia, Joanne</au><au>Senewiratne, Jamie</au><au>Bard, Frederic</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>CIL:13577, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library</title><date>2021</date><risdate>2021</risdate><abstract>Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of Helix Pomatia Lectin (HPL) (green) from the Golgi. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HPL indicates distribution of GalNac-Ts. Inactive Src is highly enriched at the Golgi. Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100) prior to staining with Hoechst (blue) and Alexa 647-conjugated-HPL. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with either a 60x objective (U Plan Super Apochromatic, UPLSAPO; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 7F in J Cell Biol. 189: 843-858. 2010. Images in Fig 7 include CIL#s 13571, 13572, 13573, 13574, 13575, 13576, 13577.</abstract><pub>UC San Diego Library Digital Collections</pub><doi>10.6075/j0mc8xqf</doi><oa>free_for_read</oa></addata></record> |
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| subjects | cervical carcinoma COPI coating of Golgi vesicle epithelial cell Golgi organization Golgi stack GTPase activity HeLa Homo sapiens integral to membrane nucleus protein binding retrograde vesicle-mediated transport, Golgi to ER small GTPase mediated signal transduction |
| title | CIL:13577, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library |
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