Fig. 4 in Isolation of an archaeon at the prokaryote eukaryote interface

Fig. 4 | Phylogeny of MK-D1 and catabolic features of Asgard archaea. a, Maximum-likelihood tree (100 bootstrap replicates) of MK-D1 and select cultured archaea,eukaryotes and bacteria based on 31 ribosomal proteins that are conserved across the three domains (Supplementary Tables 7,8). Bootstrap values around critical branching points are also shown.We used 14,024 sites of the alignment for tree construction.b, The presence or absence of amino acid degradation,electron metabolism,fermentation,C1 metabolism,sulfur metabolism and aerobic respiration in individual genomes are shown (complete pathway,full circle; mostly complete pathway,half circle).For amino acid metabolism,pathways that are exclusively used for catabolism or degradation are in bold.Glycine metabolism through pyruvate (top) or formate (bottom). Butyrate metabolism is reversible (fermentation or β-oxidation); however,butyryl-CoA dehydrogenases tend to be associated with EtfAB in the genomes,suggesting formation of an electron-confurcating complex for butyrate fermentation.Propionate was determined by the presence of methylmalonyl-CoA decarboxylase,biotin carboxyl carrier protein and pyruvate carboxylase.Propionate metabolism is also reversible;however,no member of the Asgard archaea encodes the full gene set for syntrophic degradation.Alcohol dehydrogenases can have diverse substrate specificities. See Supplementary Note 5 for abbreviations.