Data for: PIEZO1-HaloTag hiPSCs: bridging molecular, cellular and tissue imaging
PIEZO1 channels play a critical role in numerous physiological processes by transducing diverse mechanical stimuli into electrical and chemical signals. Recent studies underscore the importance of endogenous PIEZO1 activity and localization in regulating mechanotransduction. To enable physiologicall...
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| creator | Bertaccini, Gabriella Evans, Elizabeth Nourse, Jamison Dickinson, George Liu, Gaoxiang Casanellas, Ignasi Seal, Sayan Ly, Alan Holt, Jesse Yan, Shijun Hui, Elliot Panicker, Mitradas Upadhyayula, Srigokul Parker, Ian Pathak, Medha |
| description | PIEZO1 channels play a critical role in numerous physiological processes
by transducing diverse mechanical stimuli into electrical and chemical
signals. Recent studies underscore the importance of endogenous PIEZO1
activity and localization in regulating mechanotransduction. To enable
physiologically and clinically relevant human-based studies, we
genetically engineered human induced pluripotent stem cells (hiPSCs) to
express a HaloTag fused to endogenous PIEZO1. Combined with
super-resolution imaging, our chemogenetic approach allows precise
visualization of PIEZO1 in various cell types. Further, the PIEZO1-HaloTag
hiPSC technology allows non-invasive monitoring of channel activity via
Ca2+-sensitive HaloTag ligands, with temporal resolution approaching that
of patch clamp electrophysiology. Using lightsheet imaging of
hiPSC-derived neural organoids, we also achieve molecular scale PIEZO1
imaging in three-dimensional tissue samples. Our advances offer a novel
platform for studying PIEZO1 mechanotransduction in human cells and
tissues, with potential for elucidating disease mechanisms and development
of targeted therapeutics. |
| doi_str_mv | 10.5061/dryad.w6m905qwm |
| format | Dataset |
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by transducing diverse mechanical stimuli into electrical and chemical
signals. Recent studies underscore the importance of endogenous PIEZO1
activity and localization in regulating mechanotransduction. To enable
physiologically and clinically relevant human-based studies, we
genetically engineered human induced pluripotent stem cells (hiPSCs) to
express a HaloTag fused to endogenous PIEZO1. Combined with
super-resolution imaging, our chemogenetic approach allows precise
visualization of PIEZO1 in various cell types. Further, the PIEZO1-HaloTag
hiPSC technology allows non-invasive monitoring of channel activity via
Ca2+-sensitive HaloTag ligands, with temporal resolution approaching that
of patch clamp electrophysiology. Using lightsheet imaging of
hiPSC-derived neural organoids, we also achieve molecular scale PIEZO1
imaging in three-dimensional tissue samples. Our advances offer a novel
platform for studying PIEZO1 mechanotransduction in human cells and
tissues, with potential for elucidating disease mechanisms and development
of targeted therapeutics.</description><identifier>DOI: 10.5061/dryad.w6m905qwm</identifier><language>eng</language><publisher>Dryad</publisher><subject>Biological sciences ; Biophysics ; Cell biology ; Cellular imaging ; FOS: Biological sciences ; Membrane biophysics</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0009-0004-8897-173X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1894</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.5061/dryad.w6m905qwm$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Bertaccini, Gabriella</creatorcontrib><creatorcontrib>Evans, Elizabeth</creatorcontrib><creatorcontrib>Nourse, Jamison</creatorcontrib><creatorcontrib>Dickinson, George</creatorcontrib><creatorcontrib>Liu, Gaoxiang</creatorcontrib><creatorcontrib>Casanellas, Ignasi</creatorcontrib><creatorcontrib>Seal, Sayan</creatorcontrib><creatorcontrib>Ly, Alan</creatorcontrib><creatorcontrib>Holt, Jesse</creatorcontrib><creatorcontrib>Yan, Shijun</creatorcontrib><creatorcontrib>Hui, Elliot</creatorcontrib><creatorcontrib>Panicker, Mitradas</creatorcontrib><creatorcontrib>Upadhyayula, Srigokul</creatorcontrib><creatorcontrib>Parker, Ian</creatorcontrib><creatorcontrib>Pathak, Medha</creatorcontrib><title>Data for: PIEZO1-HaloTag hiPSCs: bridging molecular, cellular and tissue imaging</title><description>PIEZO1 channels play a critical role in numerous physiological processes
by transducing diverse mechanical stimuli into electrical and chemical
signals. Recent studies underscore the importance of endogenous PIEZO1
activity and localization in regulating mechanotransduction. To enable
physiologically and clinically relevant human-based studies, we
genetically engineered human induced pluripotent stem cells (hiPSCs) to
express a HaloTag fused to endogenous PIEZO1. Combined with
super-resolution imaging, our chemogenetic approach allows precise
visualization of PIEZO1 in various cell types. Further, the PIEZO1-HaloTag
hiPSC technology allows non-invasive monitoring of channel activity via
Ca2+-sensitive HaloTag ligands, with temporal resolution approaching that
of patch clamp electrophysiology. Using lightsheet imaging of
hiPSC-derived neural organoids, we also achieve molecular scale PIEZO1
imaging in three-dimensional tissue samples. Our advances offer a novel
platform for studying PIEZO1 mechanotransduction in human cells and
tissues, with potential for elucidating disease mechanisms and development
of targeted therapeutics.</description><subject>Biological sciences</subject><subject>Biophysics</subject><subject>Cell biology</subject><subject>Cellular imaging</subject><subject>FOS: Biological sciences</subject><subject>Membrane biophysics</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2024</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNpjYBA3NNAzNTAz1E8pqkxM0Ss3y7U0MC0sz-VkCHBJLElUSMsvslII8HSN8jfU9UjMyQ9JTFfIyAwIdi62UkgqykxJz8xLV8jNz0lNLs1JLNJRSE7NyQGxFBLzUhRKMouLS1MVMnMTQcp4GFjTEnOKU3mhNDeDvptriLOHbgrQouTMktT4giKg0qLKeEODeJCb4sFuioe7yZh0HQApWEid</recordid><startdate>20240307</startdate><enddate>20240307</enddate><creator>Bertaccini, Gabriella</creator><creator>Evans, Elizabeth</creator><creator>Nourse, Jamison</creator><creator>Dickinson, George</creator><creator>Liu, Gaoxiang</creator><creator>Casanellas, Ignasi</creator><creator>Seal, Sayan</creator><creator>Ly, Alan</creator><creator>Holt, Jesse</creator><creator>Yan, Shijun</creator><creator>Hui, Elliot</creator><creator>Panicker, Mitradas</creator><creator>Upadhyayula, Srigokul</creator><creator>Parker, Ian</creator><creator>Pathak, Medha</creator><general>Dryad</general><scope>DYCCY</scope><scope>PQ8</scope><orcidid>https://orcid.org/0009-0004-8897-173X</orcidid></search><sort><creationdate>20240307</creationdate><title>Data for: PIEZO1-HaloTag hiPSCs: bridging molecular, cellular and tissue imaging</title><author>Bertaccini, Gabriella ; Evans, Elizabeth ; Nourse, Jamison ; Dickinson, George ; Liu, Gaoxiang ; Casanellas, Ignasi ; Seal, Sayan ; Ly, Alan ; Holt, Jesse ; Yan, Shijun ; Hui, Elliot ; Panicker, Mitradas ; Upadhyayula, Srigokul ; Parker, Ian ; Pathak, Medha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_5061_dryad_w6m905qwm3</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biological sciences</topic><topic>Biophysics</topic><topic>Cell biology</topic><topic>Cellular imaging</topic><topic>FOS: Biological sciences</topic><topic>Membrane biophysics</topic><toplevel>online_resources</toplevel><creatorcontrib>Bertaccini, Gabriella</creatorcontrib><creatorcontrib>Evans, Elizabeth</creatorcontrib><creatorcontrib>Nourse, Jamison</creatorcontrib><creatorcontrib>Dickinson, George</creatorcontrib><creatorcontrib>Liu, Gaoxiang</creatorcontrib><creatorcontrib>Casanellas, Ignasi</creatorcontrib><creatorcontrib>Seal, Sayan</creatorcontrib><creatorcontrib>Ly, Alan</creatorcontrib><creatorcontrib>Holt, Jesse</creatorcontrib><creatorcontrib>Yan, Shijun</creatorcontrib><creatorcontrib>Hui, Elliot</creatorcontrib><creatorcontrib>Panicker, Mitradas</creatorcontrib><creatorcontrib>Upadhyayula, Srigokul</creatorcontrib><creatorcontrib>Parker, Ian</creatorcontrib><creatorcontrib>Pathak, Medha</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Bertaccini, Gabriella</au><au>Evans, Elizabeth</au><au>Nourse, Jamison</au><au>Dickinson, George</au><au>Liu, Gaoxiang</au><au>Casanellas, Ignasi</au><au>Seal, Sayan</au><au>Ly, Alan</au><au>Holt, Jesse</au><au>Yan, Shijun</au><au>Hui, Elliot</au><au>Panicker, Mitradas</au><au>Upadhyayula, Srigokul</au><au>Parker, Ian</au><au>Pathak, Medha</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>Data for: PIEZO1-HaloTag hiPSCs: bridging molecular, cellular and tissue imaging</title><date>2024-03-07</date><risdate>2024</risdate><abstract>PIEZO1 channels play a critical role in numerous physiological processes
by transducing diverse mechanical stimuli into electrical and chemical
signals. Recent studies underscore the importance of endogenous PIEZO1
activity and localization in regulating mechanotransduction. To enable
physiologically and clinically relevant human-based studies, we
genetically engineered human induced pluripotent stem cells (hiPSCs) to
express a HaloTag fused to endogenous PIEZO1. Combined with
super-resolution imaging, our chemogenetic approach allows precise
visualization of PIEZO1 in various cell types. Further, the PIEZO1-HaloTag
hiPSC technology allows non-invasive monitoring of channel activity via
Ca2+-sensitive HaloTag ligands, with temporal resolution approaching that
of patch clamp electrophysiology. Using lightsheet imaging of
hiPSC-derived neural organoids, we also achieve molecular scale PIEZO1
imaging in three-dimensional tissue samples. Our advances offer a novel
platform for studying PIEZO1 mechanotransduction in human cells and
tissues, with potential for elucidating disease mechanisms and development
of targeted therapeutics.</abstract><pub>Dryad</pub><doi>10.5061/dryad.w6m905qwm</doi><orcidid>https://orcid.org/0009-0004-8897-173X</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | DOI: 10.5061/dryad.w6m905qwm |
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| language | eng |
| recordid | cdi_datacite_primary_10_5061_dryad_w6m905qwm |
| source | DataCite |
| subjects | Biological sciences Biophysics Cell biology Cellular imaging FOS: Biological sciences Membrane biophysics |
| title | Data for: PIEZO1-HaloTag hiPSCs: bridging molecular, cellular and tissue imaging |
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